摘要
目的测定传统药对枳壳厚朴中主要活性成分及连续灌胃枳壳厚朴药对7周可被慢性应激抑郁大鼠血清、胃肠及脑组织吸收的成分。方法建立UPLC-PDA测定的新方法,色谱条件为Waters Acquity UPLC BEH(R)C18色谱柱(2.1 mm×50 mm,1.7μm);流动相:乙腈(A)和0.5%醋酸水(B);梯度洗脱方法;流量0.3 m L/min,柱温40℃;检测波长300nm。同步定性测定枳壳厚朴药对中的主要7种成分及灌胃该方7周后慢性应激抑郁大鼠血清、胃肠及脑组织中的吸收成分。结果在12 min内,该药对中柚皮芸香苷、柚皮苷、橙皮苷、新橙皮苷、水合橙皮内酯、和厚朴酚及厚朴酚7种成分获得良好分离,这7种成分均可被慢性应激抑郁大鼠胃肠组织吸收,仅橙皮苷及水合橙皮内酯可被慢性应激抑郁大鼠血清及脑组织吸收。各成分的LOD在0.12×10^(-3)~1.32×10^(-3)μL/m L,信噪比(S/N)≥3。结论该方法简单、准确、灵敏,为传统药对枳壳厚朴的质量控制及其抗抑郁、促胃肠动力作用机制的深入研究奠定了分析方法学基础。
Objective It is to simultaneously qualitative determinate the absorbed compounds in blood, gastrointestinal and brain tissues by chronic unpredictable stress induced depression rats after seven weeks＇ administration Zhiqiao-Houpu herb pair （ZQHPHP）. Methods A novel UPLC -PDA method was established to simultaneously determinate the compounds in ZQHPHP and those absorbed in blood, gastrointestinal tissue and brain tissue after administration of ZHHPHP for seven weeks. The components were separated with Waters Acquity UPLC BEH（R） C18 （2.1 mm× 50 mm, 1.7 μm） with a flow rate of 0.3 mL/min at 40℃ and detected at 300 nm. The mobile phase contained A- acetonitrile and B -0.5% acetic acid with gradient elution. Results Seven compounds in ZQHPHP were well separated including narirutin, naringin, hesperidin, neohesperidin, meranzin hydrate, honokiol and magnolol within 12 minutes which could be absorbed in gastrointestinal tissue by CUSIDR. However only hesperidin and meranzin hydrate can be absorbed in blood and brain tissue by CUSIDR. LODs of component （μL/mL） were 0.12 × 10-3 -1.32 （ S/N ≥ 3 ）. Conclusion The method is quick and sensitive which lays the foundation for the quality control of the above compounds in ZQHPHP and helps to further elucidating the mechanism behind its antidepressant and prokinetic effects.
出处
《现代中西医结合杂志》
CAS
2016年第25期2741-2743,2794,共4页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
国家自然科学基金青年基金项目(81303098)
湖南省科技厅社会发展支撑计划项目(2015SK20322)
湖南省中管局一般项目(201596)
关键词
枳壳
厚朴
UPLC-PDA
抑郁
大鼠
胃肠组织
脑组织
Zhiqiao-Houpu herb pair
UPLC -PDA
depression rats
gastrointestinal and brain tissues
cerebral tissue
