摘要
目的探讨褪黑素对大鼠肝纤维的保护作用及其对转化生长因子β1(TGF-β1)表达的影响。方法选择60只雄性SD大鼠,随机分为正常组、模型组、褪黑素低剂量组及褪黑素高剂量组,每组15只。采用连续6周皮下注射CCl4方法制备肝纤维化大鼠模型。生化法测定大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性及白蛋白(ALB)含量。分光光度计法测定肝组织羟脯氨酸(Hyp)含量;HE染色及Van Gieson(VG)染色方法评价肝脏病理改变;免疫组化法测定肝组织中TGF-β1的表达。结果与模型组相比,褪黑素处理组大鼠血清ALT、AST活性明显降低(P<0.01),褪黑素高剂量组大鼠血清ALB含量明显升高(P<0.05);褪黑素处理组大鼠肝组织Hyp含量较模型组明显降低(P<0.01);褪黑素高剂量组大鼠纤维病理评分明显低于模型组(P<0.05);TGF-β1在褪黑素处理组大鼠肝脏中的表达较模型组明显较少(P<0.01)。结论褪黑素对大鼠肝纤维化有保护作用,其可能的作用机制为抑制TGF-β1的表达。
Objective To investigate the protective effects of melatonin on hepaticfibrosis in rats and its effects on the expression of transforming growth factor β1（ TGF-β1） in liver. Methods 60 male SD rats were divided randomly into the normal group,the model group and melatonin-treated groups（ 5. 0 mg / kg,10. 0 mg / kg; n = 15respectively）. The model of liver fibrosis was induced by carbon tetrachloride（ CCl4） subcutaneously for 6 weeks continuously. The serum activities of aspartate aminotransferase（ AST）,alanine aminotransferase（ ALT） and the levels of albumin（ ALB） were detected by an autoanalyzer. The levels of hydroxyproline（ Hyp） in liver were measured by spectrophotometry. Changes in liver pathology were examined by hematoxylin and eosin（ HE） staining and Van Gieson（ VG） staining. The expression of TGF-β1 in liver tissue was evaluated by immunohistochemistry. Results Compared with the model group,serumactivities of ALT and AST were decreased significantly in melatonintreated groups（ P 〈0. 01）. The levels of serum ALB were increased significantly in melatonin-treated group（ 10. 0mg / kg） compared with the model group（ P 〈0. 05）. In comparison with the model group,the levels of Hyp in liver were decreased significantly in melatonin-treated groups（ P 〈0. 01）. Pathologic grading score of melatonin-treated group（ 10. 0 mg / kg） was much lower than that of the model group（ P〈 0. 05）. The expression of TGF-β1 in liver was decreased remarkably in melatonin-treated groups compared with the model group（ P 〈0. 01）. Conclusion Melatonin protects liver fibrosis,one mechanism of which may be related to the inhibition of TGF-β1 expression in liver.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第9期1248-1252,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽高校省级科学研究项目(编号:KJ2013A155)
关键词
褪黑素
肝纤维化
转化生长因子Β1
melatonin
liver fibrosis
transforming growth factor β1
