摘要
目的探讨乳腺癌和癌旁组织中核心结合因子(RunX2)基因的表达差异及其与乳腺癌患者的临床特征和预后的关系。方法选择75例乳腺癌患者癌组织标本,所有患者术前未经放疗和化疗,采用免疫组化法检测乳腺癌组织和癌旁组织RunX2的表达情况。分析乳腺癌患者RunX2基因的表达与年龄、病理分型、术后分期、淋巴转移、肿瘤大小、雌激素受体(ER)表型、孕激素受体(PR)表型、人类表皮生长因子2(HER2)表型、ki67表达水平以及生存率的关系。结果 75例患者的癌组织中有44例乳腺癌组织高表达RunX2,31例组织低表达RunX2;而癌旁组织仅23例高表达,52例均表达较低。癌组织和癌旁组织中RunX2的表达水平差异有统计学意义(P<0.05)。RunX2基因的表达高低与患者年龄、术后分期、淋巴结转移、病理类型、PR表型、HER2表型无明显相关性,但是与患者的ER表型和ki67表达相关(P<0.05),并且高表达RunX2患者术后3年的总体生存率较低表达患者差。结论乳腺癌组织中RunX2基因高表达与患者的ER表型和临床预后呈正相关性,进一步证实了RunX2在乳腺癌发生发展中起到重要作用。
Objective To analyze the expression level of RunX2 gene in breast cancer patients and to investigate its relationship with patients＇ clinical characteristics and outcomes. Methods Tissue samples were selected from 75 breast cancer patients without radiation and chemotherapy before surgery. Immunohistochemistry was used to compare the expression level of RunX2 between cancer and para-carcinoma tissues. The relationship was analyzed between RunX2 expression and age,pathological type,postoperative stage,lymph node metastasis,tumor size,ER,PR,HER2 and ki67 expression and the overall survival rate. Results In 75 patients＇ tumor tissues,there were 44 cases with high expression of RunX2 in breast cancer,31 cases with low expression of RunX2. Meanwhile,in patients＇ adjacent tissues,there were only 23 cases of adjacent tissues with high RunX2 expression,the rest of 52 were low or negative RunX2 expression. The expression level of RunX2 in cancer tissues and para-carcinoma tissues had significantly difference（ P〈 0. 05）. The expression level of RunX2 was not correlated with age,the postoperative stage,lymph node metastasis,pathologic types,PR and HER2 expression. But high RunX2 expression was significantly associated with ER and ki67 expression. Patients with high RunX2 expression had a poorer survival rate than those with negative or low expression. Conclusion The elevated RunX2 expression may be related to breast cancer ER phenotype and clinical outcomes. It provides further evidence that this gene might have an important,context-dependent role in breast cancer.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第9期1356-1359,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81302319)
