摘要
为研究益生菌缓解食物过敏的作用机制,本实验以牛乳β-乳球蛋白(BLG)为过敏原构建小鼠食物过敏模型,灌服长双歧杆菌BBMN68(BBMN68),采用ELISA方法检测小鼠血清及细胞培养上清中抗体和细胞因子含量,流式细胞术分析小鼠体内树突状细胞(DCs)亚型及CD4+CD25+Foxp3+Treg细胞数量的变化。结果表明,BBMN68调节了BLG小鼠体内的Th1/Th2细胞失衡,缓解了过敏反应。与过敏组小鼠相比,BBMN68显著提高了派氏淋巴结DCs中CD103表达(p<0.05),并降低了CD86和MHC-II表达(p<0.05);提高了派氏淋巴结,肠系膜淋巴结和脾脏中CD4+CD25+Foxp3+Treg细胞数量,分别增加41.91%、71.16%和61.25%。分离BBMN68组小鼠派氏淋巴结的DCs与BLG过敏小鼠的CD4+T细胞共培养,发现Foxp3+Treg细胞比例显著增加,调节性细胞因子TGF-β和IL-10分泌显著增多(p<0.05)。以上结果说明BBMN68缓解小鼠牛乳β-乳球蛋白过敏的机制与其调节DCs功能,促进其介导的免疫抑制有关。
To clarify the mechanism of probiotics on alleviating food allergy symptoms,bovine β-lactoglobulin（BLG） was used as the allergen to construct a food-allergy mouse model.BALB/c mice were sensitized by oral administration of Bifidobacterium longum BBMN68（BBMN68）.Levels of cytokines and immunoglobulins in the serum and cell culture supernatant were determined by enzyme-linked immunosorbent assay（ELISA）,and the changes in the number of CD4＋CD25＋Foxp3＋Treg cells and different subsets of dendritic cells（DCs） were evaluated by flow cytometry.The results showed that BBMN68 alleviated the T-helper 1（Th1）/T-helper 2（Th2） imbalance,and reduced hypersensitivity reactions in BLG mice.Compared with the allergy group,the administration of BBMN68 significantly enhanced the expression of CD103（p 0.05） and reduced the expression of CD86 and MHC-II（p 0.05） of DCs in Peyer＇s patches（PP）.The numbers of CD4＋CD25＋Foxp3＋Treg cells in the lymphocytes from PP,mesenteric lymph node,and spleen were increased by 41.91%,71.16%,and 61.25%,respectively,compared with those in BLG mice.These DCs modulated with BBMN68 and the CD4＋T cells from BLG mice were isolated and cultured together.The results showed an increased proportion of Foxp3＋Treg cells and an elevated secretion of regulatory cytokines TGF-β and IL-10（p 0.05）.These data demonstrated that the mechanism of BBMN68 for reducing BLG allergenicity was associated with the regulation of DC functions and promotion of the DC-mediated immune suppression.
出处
《现代食品科技》
EI
CAS
北大核心
2016年第7期6-11,共6页
Modern Food Science and Technology
基金
中央在京高校重大成果转化项目“益生菌生产关键技术科技成果转化”
国家科技支撑计划“南方大城市奶牛健康养殖生产技术集成及产业化示范”(2012BAD12B08)
