摘要
以海燕体壁为原料,选取碱性蛋白酶、Alcalase酶和菠萝蛋白酶3种酶制备胶原蛋白酶解液,以对羟自由基的抑制率为指标,优选确定海燕体壁胶原蛋白的降解酶。利用正交实验优化制备具有体外抗氧化活性的胶原蛋白肽的酶解条件。结果表明,海燕体壁胶原蛋白的适宜水解酶为碱性蛋白酶和Alcalase酶。优化酶解条件分别为碱性蛋白酶酶加量5000 U/g、pH9.5和45℃条件下酶解9 h;Alcalase酶加量为3500 U/g、pH7.5和60℃条件下酶解9 h。在2种酶最佳酶解条件下所得到的胶原蛋白肽的羟自由基清除率IC_(50)分别为16.88、17.89 mg/mL。利用海燕制备胶原蛋白肽不仅可充分利用资源,且可避免其对周边环境的污染。
The collagen was extracted from the body wall of starfish Asterina pectinifera. The results showed that alkaline protease and alcalase enzymes could hydrolyze the collagen for making the peptides. The hydrolyzing factors of alkaline protease and alcalase were then optimized by orthogonal test by taken hydroxyl radical scavenging activities of these collagen peptides as indicators. The results indicated that the optimized hydrolyzing factors were enzyme added of 5000 U/g, pH value of 9.5, temperature of 45 ℃ and hydrolyzing time of 9 h for alkaline protease. Those of alcalase were enzyme added of 3500 U/g, pH value of 7.5, temperature of 60 ℃ and hydrolyzing time of 9 h, respectively. The IC_（50） value of hydroxyl radical scavenging activities were 16.88 mg/mL and 17.89 mg/mL respectively. Collagen peptides from Asterina pectinifera could be taken as antioxidant and then avoid contamination to the surrounding environment by utilizing the Asterina pectinifera.
出处
《食品科技》
CAS
北大核心
2016年第8期59-63,共5页
Food Science and Technology
基金
海洋公益性行业科研专项(201405040)
关键词
海燕
酶解工艺
胶原蛋白肽
羟基自由基
Asterina pectinifera
hydrolysis process
collagen peptide
hydroxyl radical
