摘要
选用栝楼的藤茎为原料,采用单因素试验和正交试验设计的方法,对栝楼藤茎多糖的微波辅助提取工艺进行优化;以DPPH自由基清除率、对羟自由基清除率和还原能力为指标,评价栝楼藤茎多糖的体外抗氧化活性。结果表明:微波辅助提取栝楼藤茎多糖的最佳工艺条件为:料液比为1:15(g:mL),微波处理时间为4 min,微波功率为700 W,此提取条件下栝楼藤茎多糖的平均得率为3.71%;抗氧化试验结果显示,清除DPPH自由基和羟自由基的半抑制浓度(IC_(50))分别为1.829 mg/mL和0.52 mg/mL;还原能力测定试验中,当栝楼藤茎多糖的质量浓度为2.5 mg/mL时,在700 nm下的吸光度值达到0.426。说明栝楼藤茎多糖具有抗氧化活性,具有进一步研究的价值。
The polysaccharides were extracted by microwave-assisted water extraction from rattan stem of Trichosanthes kirilowii Maxim, and the optimum conditions were determined by single factor experiments and orthogonal experiments. Moreover, the total reducing power, DPPH radical and hydroxyl radical scavenging abilities of polysaccharides form rattan stem of Trichosanthes kirilowii Maxim were conducted to determinate the antioxidant activity in vitro. The results showed that microwave-assisted extraction technology of polysaccharides in rattan stem of Trichosanthes kirilowii Maxim was： solid/liquid ratio 1：15（g：mL）, time of extraction 4 min, and microwave power 700 W, the extraction rate of polysaccharides reached up to 3.71%. As for the antioxidant test, the scavenging capacity（IC_（50）） of polysaccharides towards DPPH free radical and hydroxyl radical were 1.829 mg/mL and 0.52 mg/mL, respectively. When polysaccharides concentration was 2.5 mg/mL, the reducing power was 0.426（OD700 nm）. It showed that the polysaccharides from rattan stem of Trichosanthes kirilowii Maxim had an antioxidant activity, and it has the value of further research.
出处
《食品科技》
CAS
北大核心
2016年第8期174-179,共6页
Food Science and Technology
基金
科技部国家级星火计划项目(2015GA690260
2016/01-2018/12)
校级青年项目(15YCKLQ007)
江苏省大学生创新创业训练计划项目指导项目
关键词
栝楼藤茎
多糖
微波辅助提取
抗氧化活性
rattan stem of Trichosanthes kirilowii Maxim
polysaccharides
microwave-assisted extraction
antioxidant activity
