摘要
目的探讨胃癌组织中血管内皮生长因子C(VEGF-C)、血管内皮生长因子受体3(VEGFR-3)的表达与幽门螺杆菌(Hp)感染及临床病理特征的关系。方法采用^(13)C呼气试验及改良Giemsa法检测40例胃癌患者的Hp感染情况,采用免疫组化SP法检测40例胃癌及配对癌旁组织中VEGF-C、VEGFR-3蛋白的表达。结果胃癌患者Hp感染率为65.0%。VEGF-C、VEGFR-3蛋白在胃癌组织中的表达量分别为(8.74±0.84)×10^(-2)和(3.64±1.96)×10^(-2),在癌旁组织中为(1.23±0.38)×10^(-2)和(0.90±0.18)×10^(-2),差异均有统计学意义(P<0.05)。VEGF-C与VEGFR-3蛋白表达呈正相关(r=0.51,P<0.05)。VEGF-C、VEGFR-3蛋白的表达与肿瘤浸润深度、分化程度、淋巴结转移、TNM分期有关(P<0.05),与肿瘤大小无关(P>0.05)。VEGF-C、VEGFR-3蛋白在Hp感染阳性胃癌组织中的表达量分别为(11.72±3.07)×10^(-2)和(8.71±2.15)×10^(-2),在Hp感染阴性胃癌组织中分别为(8.48±1.11)×10^(-2)、(5.37±1.05)×10^(-2),差异均有统计学意义(P<0.05)。结论 VEGF-C、VEGFR-3表达与Hp感染和胃癌的发生、发展有关,且Hp感染可能与胃癌淋巴管生成有关。
Objective To investigate the correlation of vascular endothelial growth factor C （VEGF-C）, and vascular endo- thelial growth factor receptor 3 （VEGFR-3） with the infection of helicobacter pylori（Hp） and clinicopathological characteristics in gas- tric carcinoma. Methods The infection with Hp in 40 patients with gastric carcinoma was detected by ^13C breath test and improved Gi- emsa. The expression of VEGF-C, VEGFR-3 in 40 cases of gastric carcinoma and their adjacent tissues were detected by immunohisto- chemical SP method. Results The infection of Hp in 40 gastric carcinoma patients was 65.0%. The expressions of VEGF-C,VEGFR- 3 in gastric carcinoma tissues were （ 8.74±0. 84）×10^-2 and （ 3.64± 1.96）×10^-2, and in adjacent tissues they were （ 1.23±0. 38 ） ×10^-2 and（0. 90±0. 18）×10^-2. The expressions of VEGF-C, VEGFR-3 in gastric carcinoma were significantly higher than those of adjacent tissues （P〈0. 05）. There was a positive correlation between VEGF-C and VEGFR-3 （r = 0. 51, P〈0. 05 ）. The expressions of VEGF-C, VEGFR-3 were related to the depth of invasion, differentiation, lymph node metastasis and TNM stage（P〈0. 05）, but not with tumor size （P〉0. 05 ）. The expression of VEGF-C, VEGFR-3 in Hp-positive gastric carcinoma tissues were （ 11.72± 3.07） ×10^-2and （ 8.71 ± 2. 15 ） ×10^-2, which were higher than （ 8.48± 1.11 ） ×10^-2 and（ 5.37± 1.05 ）×10^-2 in Hp-negative gastric carcinoma tissues, with sig- nificant differences （ P〈0. 05）. Conclusion Infection of Hp and the expressions of VEGF-C, VEGFR-3 are associated with the ooccurrence and progression of gastric carcinoma. The infection of Hp may be correlated with lymphangiogenesis.
出处
《临床肿瘤学杂志》
CAS
2016年第8期708-711,共4页
Chinese Clinical Oncology
