摘要
目的探讨转录因子POU5F1在结直肠癌组织中的表达及其与临床病理特征和预后的关系。方法收集2011年1月至2013年7月142例手术切除的结直肠癌组织及86例癌旁黏膜组织存档蜡块。采用免疫组化SP法检测以上组织中POU5F1的表达情况,并分析其与临床病理特征的关系;根据随访资料分析POU5F1表达与预后的关系,采用Cox比例风险模型分析影响预后的因素。结果 POU5F1阳性表达定位于细胞核和胞质。其在结直肠癌中的高表达率为47.2%(67/142),高于癌旁黏膜组织的23.3%(20/86),差异有统计学意义(P<0.05);POU5F1表达与性别、年龄、肿瘤部位、远处转移、CEA、CA50及CA199均无关,与浸润深度、淋巴结转移、TNM分期及分化程度有关(P<0.05);结直肠癌患者中POU5F1高表达者的中位总生存时间(OS)为26.4个月,低于低表达者的33.6个月,差异有统计学意义(P<0.05)。Cox比例风险模型分析显示,浸润深度、淋巴结转移、远处转移、TNM分期和POU5F1表达为影响OS的独立因素。结论 POU5F1在结直肠癌组织中表达增高,且与浸润深度、淋巴结转移、TNM分期、分化程度及OS有关,在结直肠癌诊断及预后预测中有一定价值。
Objective To investigate the expression of transcription factor POUSF1 in colorectal cancer tissues and its rela- tionship with clinieopathological features and prognosis. Methods One hundred and forty-two cases of paraffin-embedded colorectal cancer tissues and 86 cases of paraffin-embedded adjacent mucosa tissues were collected from January 2011 to July 2013. The expres- sion of POU5F1 was measured by immunohistochemical SP method in 142 cases of colorectal cancer and 86 cases of adjacent mucosa tissues. The relationship of POUSF1 expression with cliuicopathological parameters was analyzed. According to follow-up data, the prog- nosis of patients with different expression level of POUSF1 was investigated. The factors influencing prognosis was analyzed by Cox pro- portioual hazard model. Results The positive expression of POUSF1 was localized in the nucleus and cytoplasm. The high expression rate of POU5F1 in colorectal cancer tissues was 47.2% （67/142） , higher than 23.3% （20/86） of adjacent normal mucosa tissues, and the difference was statistically significant（P〈0. 05）. The expression of POUSF1 was not related to sex, age, tumor location, dis- tant metastasis, CEA, CAS0 and CA199 of eolorectal cancer, but it was related to the depth of invasion, lymph node metastasis, TNM stage and differentiation （P〈0. 05）. The median overall survival（OS） was 26.4 months in patients with high expression, worse than 33.6 months in patients with low expression （P〈 0. 05）. Cox proportional hazard model analysis showed that the depth of invasion, lymph node metastasis, distant metastasis, TNM staging and POUSF1 expression were independent factors influencing OS. Conclusion The expression of POUSF1 in coloreetal cancer tissues is associated with the degree of invasion, lymph node metastasis, TNM stage, differentiation and OS, and it has certain value in the diagnosis and prognostic prediction of colorectal cancer.
出处
《临床肿瘤学杂志》
CAS
2016年第8期712-717,共6页
Chinese Clinical Oncology
