摘要
目的 探讨miR-449a rs112310158基因多态性与胃癌易感性的关系。方法 共纳入448例胃癌患者和452例健康对照者。miR-449a rs112310158基因型检测采用聚合酶链反应结合直接测序方法。miR-449a表达水平检测采用荧光定量PCR方法。此单核苷酸多态性位点功能由RT-PCR和免疫组化试验验证。结果 相对于对照组,胃癌病例组在吸烟者(OR=1.345,95%CI=1.028-1.761)、饮酒者(OR=1.910,95%CI=1.465-2.490)、有肿瘤家族史者(OR=4.178,95%CI=1.554-11.231)、幽门螺杆菌感染率较高者(OR=1.428,95%CI=1.098-1.857),差异均具有统计学意义。相较AA基因型,GG基因型显著增加胃癌的发病风险(OR=2.542,95%CI:1.304-4.954,P=0.005)。相较于A等位基因,携带G等位基因的个体罹患胃癌的风险升高(OR=1.279,95%CI:1.012-1.617,P=0.043)。GG型患者比AA型患者miR-449a表达水平下降。与AA基因型相比,miR-449a靶基因PRKCE在GG基因型胃癌患者中有更高的表达。结论 该研究发现miR-449a rs112310158是胃癌的遗传易感因素之一。
Objective To investigate the relationship of a genetic variant at miR-449a and the risk of gastric cancer. Methods The polymerase chain reaction (PCR) and direct sequencing were used to genotype sequence variants of miR-449a in 448 gastric cancer(GC) cases and 452 controls. The miR-449a levels were measured by quantitative real-time PCR. The function of rs112310158 was confirmed by RT-PCR and immunohistochemistry assay. Results GC cases were more likely to be ciga- rette smokers (OR= 1. 345,95% CI = 1. 028 - 1. 761 ) and alcohol drinkers (OR = 1. 910,95 % CI = 1. 465 - 2. 490), had cancer history in the first relatives (OR= 4.178,95 % CI = 1.554 - 11.231 ), and have higher infection rate of H elicobacter pylori (OR= 1. 428,95% CI= 1. 098-1. 857). Individuals carrying the GG genotypes for the rs112310158 were associated significantly with increased risk of gastric cancer comparing with those carrying wild-type homozygous AA genotypes (OR=2. 542,95% CI: 1. 304-4. 954 ,P= 0. 005). Furthermore, the G allele increased the risk of occurrence of gastric cancer compared to the A allele (OR= 1. 279,95 Y0 CI: 1. 012 - 1. 617, P = 0. 043). rs112310158 was associated with the expressionof miR-449a and its target gene. Conclusion A genetic variant at miR-449a may play an important role in the development of gastric cancer.
出处
《现代检验医学杂志》
CAS
2016年第4期14-18,共5页
Journal of Modern Laboratory Medicine