摘要
目的探讨肾E腺素对内毒素（lipopilysaccharide，LPS）所致大鼠肺损伤的保护作用及其作用机制。方法30只SD大鼠随机分为3组（每组10只）：正常对照组大鼠静脉输注生理盐水2．4ml／（kg·h）；LPS组大鼠静脉注射LPS6mg／kg后，静脉输注生理盐水2．4ml／（kg·h）；肾上腺素组大鼠静脉注射LPS6mg／kg后，静脉输注肾上腺素0．6μg/（kg·min）。在LPS注射后2h和6h通过心导管抽血，ELISA法检测血清肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6和IL-10水平，并在6h处死大鼠取肺脏组织，分别予肺组织含水量检测，HE染色观察肺组织病理学变化，以及ELISA法检测肺泡灌洗液（BALF）内TNF-α、IL-6和IL-10水平。结果（1）与正常对照组肺含水量（70．19％±5．87％）相比，LPS组肺含水量（85．24％±5．87％）明显升高（P〈0．05），与LPS组相比，肾上腺素组肺含水量（78．00％±6．41％）明显降低（P〈0．05）。（2）病理观察结果显示，LPS组大鼠肺泡隔内毛细血管充血、水肿及炎症细胞浸润，少部分肺组织可见肺不张及肺泡内水肿、出血。肾上腺素组大鼠肺病理损伤较LPS组明显减轻。（3）与LPS组相比，肾上腺素组各时点血清TNF—α及IL－6水平明显降低（P〈0．05），IL－10水平明显升高（P〈0．05）。（4）与LPS组相比，肾上腺素组大鼠BALF中TNF－α水平降低[（78±9）ng／Lvs．（102±16）ng／L]，IL－6水平降低[（268±42）ng/Lvs．（347±50）ng／L]，IL－10水平升高[（210±23）ng／Lvs．（146±34）ng/L]（P〈0．05）。结论。肾上腺素可减轻LPS诱导的急性肺损伤，其保护作用可能与降低TNF—α、IL－6水平，升高IL－10水平有关。
Objective To investigate the effects of epinephrine in sepsis-associated lung injury in rats. Methods Thirty SD rats were randomly divided into three groups （n = 10 per group） ：control group received intravenous 0. 9% saline 2.4 ml/（ kg. h ） ; LPS group received intravenous LPS （ 6 mg/kg ） ; epinephrine treatment group received an infusion of epinephrine 0.6 p.g/（ kg-min） after LPS intravenous injection. Blood samples were taken at 2 h and 6 h after LPS injection and the levels of serum tumor necrosis factor （TNF）-α,interleukin（ IL）45 and IL-10 were detected. The rats were sacrificed at 6 h. The lung tissues and bronchoalveolar lavage fluid（BALF） were collected. Pathological changes of the lung tissues were observed under light microscope. Water content of lung,expression of TNF-α,IL-6 and IL-10 in BALF and in serum were detected. Results （ 1 ） The water content of lung in LPS group significantly increased compared with that in control group （ 85.24 % ± 5.87 % vs. 70. 19 % ± 5.87 % ） and epinephrine group （ 78.00 % ± 6.41% ） （ P 〈 0. 05 ）. （ 2 ） Pathological examination showed that LPS could cause pulmonary capillary hyperemia, edema, inflammatory cells infiltration. Atelectasis and alveolar edema were found in small number of lung tissue. Compared with LPS group, epinephrine ameliorated the lung pathological injury. （3）Compared with LPS group,serum levels of TNF-α and IL-6 significantly decreased （ P 〈 0. 05 ）, whereas IL-10 increased （ P 〈 0. 05） in epinephrine group. （4） Compared with LPS group,BALF levels of TNF-α （78 ±9）ng/L vs. （ 102± 16 ） ng/L ] and IL-6 E （ 268 ± 42 ） ng/L vs. （ 347 ± 50 ） ng/L ] significantly depressed （ P 〈 0. 05 ）, whereas BALF levels of IL-10 [（210 ± 23 ） ng/L vs. （ 146 ±34 ） ng/L ] elevated （ P 〈 0.05 ） in epinephrine group. Conclusion Epinephrine could reduce the acute lung injury caused by LPS. Its protective effect may be related to decreasing the levels of TNF-α and IL-6 ,elevating IL-10 level.
出处
《中国小儿急救医学》
CAS
2016年第8期522-525,共4页
Chinese Pediatric Emergency Medicine
基金
广州市卫生和计划生育科技一般引导项目（20161A011026）
广州市科技计划项目（2014Y2-00181）
关键词
肾上腺素
脓毒症
炎症介质
急性肺损伤
大鼠
Epinephrine
Sepsis
Inflammatory mediators
Acute lung injury
Rats
