摘要
目的:研究甲状腺癌细针穿刺组织中BRMS1和Cx43的表达量及其与肿瘤恶性程度的相关性。方法:选择在我院接受甲状腺细针穿刺活检的患者进行研究,经病理学诊断后筛选甲状腺癌患者60例和甲状腺良性肿瘤患者60例,收集穿刺组织并测定BRMS1和Cx43的表达量,收集血清标本并测定Gal-3、CEACAM1、MMP2、MMP9的含量。结果:甲状腺癌组织中BRMS1和Cx43的mRNA含量及阳性表达率均显著低于甲状腺良性肿瘤组织;不同病理分型、肿瘤直径甲状腺癌组织中BRMS1、Cx43的mRNA含量无差异,TNM III-IV期甲状腺癌组织中Cx43的mRNA含量显著低于TNM I-II期甲状腺癌组织,不同TNM分期甲状腺癌组织中BRMS1的mRNA含量无差异,存在淋巴结转移的甲状腺癌组织中BRMS1、Cx43的mRNA含量显著低于无淋巴结转移的甲状腺癌组织;甲状腺癌组织中BRMS1、Cx43阳性表达患者的血清Gal-3、CEACAM1、MMP2、MMP9含量显著低于甲状腺癌组织中BRMS1、Cx43阴性表达患者。结论:甲状腺癌细针穿刺组织中BRMS1和Cx43的低表达与肿瘤的远处转移及恶性程度相关,Cx43的低表达还与肿瘤的生长以及癌细胞的增殖相关。
Objective： To study the BRMS1 and Cx43 expression in fine needle aspiration thyroid cancer tissue and their correlation with tumor malignancy. Methods： Patients undergoing thyroid fine needle aspiration biopsy in our hospital were selected for study, 60 patients with thyroid cancer and 60 patients with benign thyroid tumor were screened after pathological diagnosis, biopsy tissue was collected to determine the expression of BRMS1 and Cx43, and serum specimens were collected to determin Gal-3, CEACAM1, MMP2 and MMP9 content. Results, mRNA levels and positive expression rate of BRMS1 and Cx43 in thyroid cancer tissue were significantly lower than those in benign thyroid tumor tissue; mRNA levels of BRMS1 and Cx43 in thyroid cancer tissue with different pathological types and tumor diameters were not different, mRNA level of Cx43 in thyroid cancer tissue with TNM III-IV stage was significantly lower than that in thyroid cancer tissue with TNM I-II stage, mRNA levels of BRMS1 in thyroid cancer tissue with different TNM stages were not different, and mRNA levels of BRMS1 and Cx43 in thyroid carcinoma tissue with lymph node metastasis were significantly lower than those in thyroid carcinoma tissue without lymph node metastasis serum Gal-3, CEACAM1, MMP2 and MMP9 levels in patients with positive BRMS1 and Cx43 expression in thyroid cancer tissue were significantly lower than those in patients with negative BRMS1 and Cx43 expres- sion in thyroid cancer tissue. Conclusion.. Lower expression of BRMS1 and Cx43 in fine needle aspiration thyroid cancer tissue is associated with the distant metastasis and malignant degree of tumor, and lower expression of Cx43 is also associated with the growth of tumor and cancer cell proliferation.
出处
《海南医学院学报》
CAS
2016年第17期2032-2034,2038,共4页
Journal of Hainan Medical University
基金
上海市卫计委青年项目(20144Y0199)~~
