摘要
【摘要】目的探讨单核细胞趋化因子-1（MCP-1）及白介素石（IL-6）在眼球穿孔伤患者房水中的表达水平及其意义。方法眼球穿孔伤30例（30只眼）作为试验组，在眼球穿孔伤行-期缝合术后10d抽取房水200μl；单纯年龄相关性白内障30例（30只眼）作为正常对照组，在行超声乳化吸出术术中抽取200μl房水。所有房水样本均用酶联免疫吸附试验（ELISA）测定MCP-1及IL-6水平。用t检验比较两组房水标本中MCP-1、IL-6浓度。结果试验组房水标本中MCP-1、IL-6浓度分别为（8103．18±1961．76）pg／ml和（161．35±37．34）pg／ml；对照组房水标本中MCP-1、IL-6浓度分别为（217．98±158．32）μg／ml和（2．48±5．19）pg／ml。试验组房水标本中MCP-1、IL-6表达水平均高于正常对照组，差异有统计学意义（MCP-1：F=31．901，P=0．000，IL-6：F=29．302，P=0．000）。结论眼球穿孔伤后房水中MCP-1和IL-6表达水平明显升高，这提示MCP-1及IL-6可能是眼球穿孔伤后眼内炎症反应及其相关并发症的重要介质，对眼球穿孔伤术后炎症和相关并发症的治疗有指导意义。
Objective To investigate the level of monocyte chemoattractant protein-1 （MCP-1） and interleukin-6 （1L-6） in the aqueous humor of patients with perforating injury of eyeball. Methods Thirty eyes of 30 patients with perforating injury of eyeball were recruited as the clinical trial group. Thirty eyes of 30 patients with age-related cataract were recruited as the control group. Aqueous humor samples were collected in the ocular trauma patients at 10 days after their first repairment. The control group underwent phacoemulsification cataract surgeries and aqueous humor was taken during the surgery. MCP-1 and IL-6 concentrations were detected by enzyme-linked immunosorbent assay （ELISA）. T test was used to compare the differences of MCP-1 and IL-6 concentrations between the two groups. Results The differences were statistically significant in MCP-1 and IL-6 levels between the two groups （MCP-1 ： F = 31. 901, P = 0. 000; IL-6 ： F = 29. 302, P = 0. 000）. Patients with perforating injury of eyeball had higher levels of MCP-1 （8103.18 ± 1961.76） pg/ml and IL-6 （161.35 ±37.34） pg/ml compared with the control group, MCP-1 （217.98 ±158.32）pg/ml and IL-6 （2.48 ±5.19）pg/ml. Conclusion Patients with perforating injury of eyeball have higher level of MCP-1 and IL-6 in the aqueous humor. These inflammatory markers may play roles in the post-operative inflammation and may be associated with complications.
出处
《中华眼外伤职业眼病杂志》
2016年第8期561-564,共4页
Chinese Journal of Ocular Trauma and Occupational Eye Disease
基金
基金项目：国家自然科学基金（81260151）
基金项目：云南省科技计划项目（2014RA009）
