摘要
以正常和腐败的产品为材料研究引起真空包装肘花胀袋的微生物,将传统培养与分子生物学方法(16S r DNA序列分析和PCR-DGGE技术)相结合对分离纯化的微生物进行鉴定,并将分离到的细菌进行回接验证试验。结果表明,比较从正常和胀袋产品中分离纯化到的14株和18株菌发现,胀袋组中含有对照组没有的细菌。16S r DNA的分析结果显示这些菌株主要是芽孢杆菌[枯草芽孢杆菌(Bacillus subtilis)、地衣芽孢杆菌(Bacillus licheniformis)]以及寡养单胞菌(Stenotrophomonas maltophilia和Stenotrophomonas pavanii);而PCR-DGGE分析结果表明这些菌株主要是包括产气荚膜梭茵(Clostridium perfringens)在内的梭状芽孢杆菌(Clostridium sp.)和假单胞菌(Pseudomonas sp.),将分离到的细菌回接到样品中,产品即发生胀袋,可见,引起肘花胀袋的微生物是芽孢杆菌和寡养单胞菌。
Using accepted product and swollen bag of pork jelly as materials,the microorganisms of swollen bag of pork jelly were studied. The isolation and purification microorganisms were isolated by traditional and molecular methods （16S rDNA analysis, and PCR-DGGE technology）, and these strains were inoculated to the product to verify the result. According to the results, some bacteria existed in package swollen pork jelly group but couldn＇t be found in control group, comparing the total of 14 and 18 strains from the control group and package swollen pork jelly group.The results showed these bacteria included Bacillussp. （Bacillus subtilis and Bacillus licheniformis） and Stenotrophomonas sp. （Stenotrophomona smaltophilia and Stenotrophomona spavanii） by 16SrDNA analysis, and included Clostridium sp.（including Clostridium pesfringens） and Pseudomonas sp. by PCR-DGGE technology. The isolated strains were inoculated to the pork jelly,and the swollen bag was generated except the control group. It was verified to be the strains causing the bag swollen through an artificial infection test.
出处
《湖北农业科学》
2016年第13期3434-3438,共5页
Hubei Agricultural Sciences
基金
国家科技支撑计划项目(2014BAA03B05)
四川省科技支撑项目(2015SZ0215)
