摘要
目的 探讨β1,3-N-乙酰氨基葡萄糖转移酶-8（β3GuT8）在人垂体腺瘤中的表达及其意义.方法 收集经手术和病理检查结果证实的垂体腺瘤标本58例,其中侵袭组32例,非侵袭组26例,分别采用免疫组织化学和Western blot方法检测侵袭性和非侵袭性垂体腺瘤组β3GnT8、相对高分子质量细胞外基质金属蛋白酶诱导剂（HG-CD147）、基质金属蛋白酶-2（MMP-2）的表达.结果 免疫组织化学结果显示33 GnT8在侵袭组的阳性率为87.5％,明显高于非侵袭组中的57.7％（P＜0.05）;Western blot结果显示β3GnT8、HG-CD147、MMP-2在侵袭组表达水平均明显高于非侵袭组（t=2.72,P＜0.05;t =2.81,P＜0.05;t =2.68,P＜0.05）,且随着β3GnT8表达量增加,HG-CD147及MMP-2的表达量亦增加,Pearson相关分析显示在侵袭组中β3GnT8、HG-CD147、MMP-2表达两两呈正相关（r =0.78,P ＜0.05;r=0.64,P＜0.05;r=0.52,P＜0.05）,在非侵袭组中无明显相关（P＞0.05）.结论 β3GnT8在垂体瘤中表达且和垂体腺瘤的侵袭性密切相关。
Objective To explore the expression of β1,3-N-acetylglucosaminyltransferase 8 （β3GnT8） in pituitary adenomas and its influence on the invasive behaviors.Methods A total of 58 cases of pituitary adenoma,including 32 cases in invasive group and 26 cases in non-invasive group,were collected.The expression of β3GnT8,high glycoform of CD147 （HG-CD147） and matrix metalloproteinase （MMP）-2 was determined by immunohistochemical method and Western blotting.Results The β3GnT8 expression rate was significantly higher in the invasive group than in the non-invasive group （87.5% vs.57.5%,P 〈0.05）.Western blotting revealed that the expression of β3GnT8,HG-CD147 and MMP-2 in the invasive group was significantly increased as compared with the non-invasive group （t =2.72,P 〈 0.05;t = 2.81,P 〈 0.05;t =2.68,P 〈 0.05）,and with the increased expression of β3GnT8,the expression of HG-CD147 and MMP-2 also increased.There were mutually positive correlations among β3GnT8,CD147 and MMP-2 expression in invasive pituitary adenomas （r =0.78,P 〈 0.05;r =0.64,P 〈 0.05;r =0.52,P 〈 0.05）.Conclusion The β3GnT8 expression might relate with the aggressiveness and invasion of pituitary adenomas.It may be an useful maker for predicting invasiveness and provides a new way for the diagnosis and treatment of invasive pituitary adenomas.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2016年第9期2100-2102,共3页
Chinese Journal of Experimental Surgery
基金
山东省科技发展计划（2010GSFl0225）
济南市科技发展计划（201303038）
