摘要
目的:研究高迁移率族蛋白B1(HMGB1)、基质金属蛋白酶-9(MMP-9)在胎膜早破(PROM组)合并绒毛膜羊膜炎(CAM)患者胎膜组织中的表达及意义。方法:选取足月PROM剖宫产患者30例为研究组,足月择期剖宫产妊娠妇女30例为对照组。取胎膜组织行病理检查,按病理结果再分为合并和非合并CAM组。采取免疫组化法和实时荧光定量PCR法检测PROM组和对照组孕妇胎膜组织中HMGB1、MMP-9蛋白及mRNA表达。结果:PROM组的CAM发生率为56.67%(17/30),高于对照组[13.33%(4/30)],差异有统计学意义(P<0.05)。PROM组和对照组中,合并CAM者胎膜组织中HMGB1、MMP-9蛋白和mRNA相对表达量均高于未合并CAM者,差异有统计学意义(P<0.05)。PROM组胎膜组织中HMGB1和MMP-9 mRNA表达呈正相关(r=0.439,P<0.05)。结论:HMGB1和MMP-9表达可能与PROM发生、发展相关。
Objective： To explore the expression and the meaning of high mobility group protein B1（ HMGB1） 、matrix metalloproteinases-9（ MMP-9） in the fetal membranes of PROM（ PROM group） CAM patients. Methods： We chose a total of 30 full-term pregnant women with PROM（ PROM group） and 30 cases without PROM（ control group） underwent term cesarean delivery. Women after childbirth underwent pathological diagnosis of chorioamnionitis in fetal membranes. According to the pathological examination were then divided into combined CAM group and unincorporated CAM group. Used immunohistochemistry and real-time fluorescent quantitative PCR methods to detect the protein and mRNA expression level of HMGB1、MMP-9 in the fetal membranes of PROM group and control group. Results： The CAM incidence of PROM was 56. 67%（ 17 /30）,higher than those of the control group[13. 33%（ 4 /30） ],the difference was statistically significant（ P〈 0. 05）. The protein and mRNA expression level of HMGB1、MMP-9 in the fetal membranes of PROM group and control group,combined CAM membranes were relatively higher than that of unincorporated CAM,the difference was statistically significant（ P〈 0. 05）. The mRNA expression level of HMGB1、MMP-9 in the fetal membranes of PROM group were positively correlated（ r = 0. 439,P 〈0. 05）. Conclusion： The expression of HMGB1、MMP-9 may be associated with PROM occurrence and development.
出处
《现代妇产科进展》
CSCD
北大核心
2016年第8期582-585,共4页
Progress in Obstetrics and Gynecology
关键词
胎膜早破
绒毛膜羊膜炎
高迁移率族蛋白B1
基质金属蛋白酶-9
胎膜组织
Premature rupture of membranes
Chorionic membrane
High mobility group protein B1
Matrix metalloproteinase-9
Fetal membranes
