摘要
以nos Z基因为标记,应用聚合酶链式反应-变性梯度凝胶电泳法（PCR-DGGE）对牛粪堆肥过程中反硝化细菌群落结构进行研究,通过冗余分析（Redundancy analysis,RDA）法,分析反硝化细菌群落结构变化与堆肥过程中理化参数（堆体温度、pH、含水率、氨态氮和硝态氮）之间的关系。结果表明,反硝化细菌群落的Shannon-Weaver指数在堆肥0~4 d升高,在7~29 d减小。对优势条带进行测序发现Alcaligenes存在于整个堆肥过程中,判定其为此过程中优势菌属。反硝化细菌群落结构在堆肥前期（0~4 d）变化较小,而在降温腐熟期（23~29 d）变化剧烈。皮尔逊相关系数分析表明,反硝化细菌群落多样性与含水率呈极显著正相关关系（r=0.960,P〈0.01）,与温度呈显著正相关关系（r=0.766,P〈0.05）。
In the present paper, the community structure of denitrifying bacteria during cow manure composting process was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） based on the nos Z gene. The relationship between the changes of denitrifying bacteria community structure and the physic-chemical parameters（temperature, pH, water content, NH＋4-N and NO-3-N）was evaluated by redundancy analysis（RDA）. Results showed that the Shannon-Weaver index of denitrifying bacteria increased during 0~4d but decreased during 7~29 d of the composting. Based on the sequencing of dominant bands, Alcaligenes were the dominant species during composting. Redundancy analysis（RDA）showed that the community structure of dinitrifying bacteria changed little at the early stage of composting（0~4 d）, while it changed dramatically in the maturity stage of composting（23~29 d）. Pearson correlation analysis showed that denitrifying bacteria community diversity was extremely significantly positively correlated with water content（r=0.960, P≤0.01）, and significantly positively correlated with temperature（r=0.766, P≤0.05）.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2016年第9期1781-1787,共7页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(31272484
31372351)
关键词
堆肥
反硝化细菌
理化参数
群落结构
冗余分析
compost
denitrifier
physico-chemical parameter
bacterial community structure
redundancy analysis
