摘要
目的研究中药金骨莲胶囊对兔膝骨关节炎(OA)关节软骨中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达水平的影响,探讨其可能作用机制。方法 40只家兔分为4组:空白对照组(10只)、模型对照组(10只)、金骨莲胶囊组(10只,74.4mg·kg-1·d-1)、氨基葡萄糖组(10只,150mg·kg-1·d-1)。除空白对照组外其余均按改良Hulth法复制OA模型,术后当天开始药物灌胃,对照组仅灌服等量生理盐水,1次/天,连用8周。8周后处死家兔取右膝股骨髁及胫骨平台大体观察。采用Mankin评分评价关节软骨退变情况;采用实时荧光定量PCR(RT-qPCR)法检测股骨内侧髁负重区关节软骨IL-1β、IL-6和TNF-α的mRNA相对表达量。结果空白对照组、金骨莲胶囊组、氨基葡萄糖组和模型对照组Mankin评分分别为(1.380±0.183)、(2.580±0.464)、(5.250±0.143)、(10.380±0.183)分,Mankin评分依次升高,组间比较差异均有统计学意义(P<0.05)。金骨莲胶囊组和氨基葡萄糖组3种炎性因子mRNA表达量均明显低于模型对照组,差异均有统计学意义(P<0.05);中药金骨莲胶囊在下调IL-1βmRNA方面与氨基葡萄糖作用相当,差异无统计学意义(P=0.271)。金骨莲胶囊在降低IL-6和TNF-αmRNA方面优于氨基葡萄糖作用,差异均有统计学意义(P<0.05)。结论中药金骨莲胶囊可不同程度下调关节软骨中IL-1β、IL-6和TNF-α炎症因子的表达,使软骨退变的部分因素得到控制,从而起到保护关节软骨的作用。
Objective Study of Golden Lotus capsule and psammosilene tun^co^des protective of knee osteoarthritis in domes- tic rabbits. Methods A total of 50 healthy domestic rabbits were randomly and equally divided into A,B,C,D,E group,group A was the control group, group B： model control group, group C to give the drug of Psammosilene tunicoides,D group to give Golden Lotus capsule,group E to give the glucosamine drug（n=10）. Except for A group,the other 4 groups of domestic rabbits were in- duced right knee osteoarthritis models by the modified Hulth＇s modeling method. After modeling, the experimental group began tak- ing the drug,A and B group were fed with normal saline,once a day. All rabbits were kills by gas embolism at the eighth week. The articular cartilage .of medial malleolus of femur split into two and the right knee joints were obtained. One hematoxylin eosin staining and safranin o staining Mankin score of articular cartilage,the other with real-time fluorescent quantitative PCR technique to detect articular cartilage of IL-1β,IL-6 and TNF-α of the mRNA relative expression level. Results Mankin score of group A,B,D and E were（1.38±0. 183） ,（2.58±0. 464） ,（5.25±0. 143）and（10.38±0. 183）respectively,and it increased in order（P〈0.05）, mRNA expression of group D and E were significantly lower than group B（P〈0.05）. Psammosilene tunicoides, Golden Lotus capsule and glucosamine have different levels of protection effect for IL-1β,IL-6 and TNF-α in the articular cartilage of medial malleolus of fe- mur. Compared with control group, the difference between Psammosilene tunicoides and Golden Lotus capsule groupthe was statisti- cally significant （P〈0.05）. Conclusion Psammosilene tunicoides and Golden Lotus capsule could decrease the expression of IL-1β, TNF-α and IL-6 in different degree.
出处
《重庆医学》
CAS
北大核心
2016年第26期3611-3615,共5页
Chongqing medicine
基金
贵州省科技厅社发攻关基金资助项目(黔科合SY[2010]3091号)
硕士启动基金项目(院字[2013]20号)
