摘要
目的:研究跌打通痹膏对兔膝骨关节炎关节软骨细胞MMP-1,MMP-3,MMP-13及COL-Ⅱ的mRNA表达的影响,探讨跌打通痹膏干预软骨基质降解的作用机制。方法:新西兰兔60只随机分成空白组、假手术组、模型组、跌打通痹膏组、骨通贴组,每组12只。空白组不做任何处理,模型组、跌打通痹膏组、骨通贴组采用改良Hulth造模法,假手术组打开关节腔后不做处理,直接缝合。正常组、假手术组不予处理,模型组、跌打通痹膏组、骨痛贴组分别用胶带、跌打通痹膏、骨痛贴膏贴敷治疗4周。术后第5周各组随机选择1只兔处死验证造模,进行膝关节软骨形态肉眼观察和切片光镜观察,检测关节软骨中MMP-1,MMP-3,MMP-13及COL-Ⅱ的mRNA表达。结果:1)膝关节软骨形态肉眼观察和切片光镜观察:模型组与空白组有差异,跌打通痹膏组、骨通贴组与模型组比较有差异,跌打通痹膏组与骨通贴组比较无差异。2)MMP-1,MMP-3,MMP-13和COL-Ⅱ的mRNA表达检测:模型组与空白组比较,MMP-1,MMP-3及MMP-13的mRNA表达显著提高,差异有统计学意义(P<0.01),COL-ⅡmRNA表达显著降低,差异有统计学意义(P<0.01);跌打通痹膏组、骨通贴组与模型组比较,MMP-1,MMP-3及MMP-13的mRNA表达显著降低,差异有统计学意义(P<0.01),COL-ⅡmRNA表达显著升高,差异有统计学意义(P<0.05);跌打通痹膏组与骨通贴组比较,MMP-1,MMP-3,MMP-13及COL-Ⅱ的mRNA表达差异无统计学意义(P>0.05)。结论:1)跌打通痹膏和骨通贴对KOA均有治疗作用,可延缓KOA的病程,且两者间差异无统计学意义。2)跌打通痹膏通过抑制MMP-1,MMP-3及MMP-13的mRNA表达,减缓COL-Ⅱ降解,从而有效保护膝骨关节炎模型兔关节软骨,延缓关节软骨退变。
Objective：To observe the effect of Dieda Tongbi emplastrum on expression of MMP-1,MMP-3,MMP-13 and COL-ⅡmRNA in articular cartilage cells of rabbit with knee osteoarthritis,and to explore its mechanism on the degradation of cartilage matrix.Methods：All 60 New Zealand rabbits were randomly divided into blank group,sham-operated group,modeling group,Dieda Tongbi emplastrum group,and gutong emplastrum group（n=12）.The knee osteoarthritis model were established by improved Hulth molding method in modeling group,Dieda Tongbi emplastrum group,and Gutong emplastrum,while one rabbits was selected randomly to confirm the successful model 5weeks after operation.They were treated with tape fixed,Dieda Tongbi emplastrum,and Gutong emplastrum respectively in above threes groups for 4weeks.The joint cavities were opened without any treatment and sutured directly in the sham-operated group.There was not any treatment in the blank group.Then the knee joint cartilage was observed by eyes and microscopy.The expression of MMP-1,MMP-3,MMP-13 and COL-ⅡmRNA in articular cartilage cells were detected.Results：1）There was significant difference between blank group and model group in the knee joint cartilage observation by eyes and microscopy（P〈0.05）.Compared with model group,Dieda Tongbi emplastrum group and Gutong emplastrum group were significant difference（P〈0.05）,and there was no significant difference between Dieda Tongbi emplastrum group and Gutong emplastrum group（P〈0.05）.2）The expression of MMP-1,MMP-3and MMP-13 in the model group was increased significantly than blank group（P〈0.01）,but the COL-ⅡmRNA was decreased significantly（P〈0.01）.The expression of MMP-1,MMP-3and MMP-13 in the Dieda Tongbi emplastrum group and Gutong emplastrum group was decreased significantly than model group（P〈0.01）,but the COL-ⅡmRNA was increased significantly（P〈0.05）,and there was no significantly difference between Dieda Tongbi emplastrum group and Gutong emplastrum group（P〈0.05）.Conclusions：Dieda Tongbi emplastrum and Gutong emplastrum are both coequal effective for treating of knee osteoarthritis.Dieda Tongbi emplastrum protects articular cartilage degeneration by inhibiting of MMP-1,MMP-3,and MMP-13 mRNA expression,and reducing the COL-Ⅱdegradation.
出处
《中国中医骨伤科杂志》
CAS
2016年第9期1-4,10,共5页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
湖南省教育厅科研项目-优秀青年项目(13B085)
关键词
膝骨性关节炎
跌打通痹膏
基质金属蛋白酶
Ⅱ型胶原
knee osteoarthritis
Dieda Tongbi emplastrum
MMP-1mRNA
MMP-3mRNA
MMP-13mRNA
COL-ⅡmRNA
