摘要
为提高籼碎米蛋白质碱法提取效率,在Min-Run ResⅣ析因设计和爬陡坡试验的基础上,利用二次旋转中心组合响应面优化方法研究碱液浓度、籼碎米粒度、固液比对大米蛋白质提取的影响规律,并优化其提取工艺条件。结果表明,籼碎米蛋白质最优碱法提取工艺为碱液浓度O.09 mol/L,籼碎米粒度80目,固液比1:13.5(g/mL),碱提温度50℃,碱提时间120 min。该条件下蛋白质提取率为(82.25±2.53)%,大米蛋白质产物纯度为(91.68±1.26)%。
To improve the efficiency of protein extraction from broken long-grain rice, a quadratic rotatable central composite response surface methodology （qrCCD-RSM） was utilized to investigate the affecting mechanism of alkali concentrations, broken long-grain rice particle sizes and solid liquid ratios （SLR）, based on the results of Min-Run Res IV factorial design and steep accent experiments （SAE）. The optimal extraction condition was obtained, i.e. 80 mesh of broken long-grain rice was used to extrat proteins, soluting in 0.09 mol/L of NaOH buffer, with the SLR 1 g ： 13.5 mL at 50℃ for 120 min. Under this optimized condition, the protein extraction rate and its purity was found to be （82.25±2.53）% and （91.68±1.26） %, respectively.
出处
《食品与机械》
CSCD
北大核心
2016年第8期173-177,共5页
Food and Machinery
基金
湖南省高校科研项目(编号:12C1049)
国家自然科学基金(编号:31050012)
湖南化工职业技术学院院级课题(编号:HNHY2015002)
关键词
籼碎米
蛋白质
碱法提取
broken long-grain rice
protein, alkaline extraction
