摘要
为提高药用成分皂苷的提取得率,合理有效开发七叶一枝花药材,以乙醇浓度、温度、料液比和提取时间为单因素进行试验,确定各因素的最佳水平。在该基础上,采用基于Box-Behnken试验设计的响应面法进一步优化,得到最佳提取工艺为:乙醇浓度39%、水浴温度86℃、提取时间5.5min。在该条件下,七叶一枝花中皂苷提取得率达11.02%。内部沸腾法提取七叶一枝花中皂苷具有简便、快速、高效等优点。
In order to improve the unit yields of medicinal compo- nents, and provide a scientific basis for effective exploring of Rhizoma paridis, single factor experiments were combined with inner ebullition and response surface methods to extract the saponin from Rhizoma paridis. Based on the single factor experiments of ethanol concentration, temperature, liquid feed ratio, and extraction time, the best levels of all factors were established. The extraction conditions were optimized by the Box-Behnken design and the optimum conditions were as follows: 39% of ethanol concentration, 86℃ of extraction temperature, and 5.5 rain of exlraction time. The yield of saponin extracted under the optimal conditions reached 11.02%. The present strategy is simple, rapid and efficient.
出处
《食品与机械》
CSCD
北大核心
2016年第8期178-181,共4页
Food and Machinery
基金
湖南省科技计划项目(编号:2014NK3002)
湘南优势植物资源综合利用湖南省重点实验室开放基金(编号:XNZW14C05)
永州市科技局项目(编号:2015[10])