摘要
目的探讨子痫前期患者生化指标及分子机制。方法收集2011年3月至2015年12月48例子痫前期轻度患者,32例子痫前期重度患者,以及50例正常妊娠妇女血清及胎盘,检测血清中胎盘生长因子（placenta growth factor,PLGF）,可溶性血管内皮生长因子受体1（soluble fms-like tyrosine kinase-1,s Flt-1）的水平,以及分析胎盘中活性氧（reactive oxygen species,ROS）与抗氧化基因的表达情况。结果在孕12~16周、孕26~30周及孕37~41周,轻度与重度组PLGF水平均显著低于正常妊娠组水平（P〈0.05）;在孕26~30周及孕37~41周,轻度与重度组s Flt-1水平显著高于正常妊娠组水平（P〈0.05）;轻度与重度组胎盘氧化应激明显增强,表现为ROS水平增加及抗氧化应激基因的上调。结论孕妇血清中PLGF和s Flt-1水平变化与子痫前期相关;ROS异常增加是子痫前期发生的分子基础。
Objective To explore the biomarkers and molecular mechanism of preeclampsia. Methods Serums and placentas from 48 cases of mild preeclampsia, 32 cases of severe preeclampsia and 50 cases of normal pregnancies were collected during March 2011 to December 2015 for detecting the level of PLGF（placenta growth factor） and s Flt-1（soluble fms-like tyrosine kinase-1） in serums and ROS and antioxidant genes in placentas. Results Maternal serum PLGF levels during all periods were significantly lower in the preeclamptic women than in normal controls（P〈0.05）.The level of maternal plasma s Flt-1 was significant higher in the preeclamptic women than that in normal controls during the latter two periods of pregnancy（P〈0.05）. Compared with normal controls, preeclamptic women showed elevated ROS and up-regulated antioxidant genes expression. Conclusion Maternal plasma PLGF and s Flt-1were associated with the development of preeclampsia, and ROS might play as a molecular basis for preeclampsia.
出处
《中国妇产科临床杂志》
CSCD
北大核心
2016年第5期428-430,共3页
Chinese Journal of Clinical Obstetrics and Gynecology
