摘要
建立了双波长高效液相色谱法（RP-HPLC）同时测定自制植物防晒复配物中绿原酸、芦丁、黄芩苷、丹皮酚、黄芩素、大黄素和大黄酚7种防晒成分的含量。样品用纯甲醇溶解,采用Wonda Sil C18WR色谱柱（250 mm×4.6 mm,5μm）分离,以甲醇和0.1%磷酸溶液为流动相进行梯度洗脱,流速1.0 m L/min,检测波长270和327 nm,柱温40℃。结果表明,绿原酸、芦丁、黄芩苷、丹皮酚、黄芩素、大黄素和大黄酚的线性范围分别为4.24~42.4,6.76~67.6,4.16~41.6,4.90~49.0,4.34~43.4,4.28~42.8和5.72~57.2 ng,线性相关系数均大于0.999。7种防晒成分的平均回收率为92.4%~107.8%,RSD为3.81%~7.38%。该方法操作简便、准确、重复性好,可用于自制植物防晒复配物的质量控制。
A RP - HPLC method was established for simultaneous determining chlorogenic acid, lutin, baicalin, paeonol, baicalein, emodin and chrysophanol in blend of plant extract. The sample was extracted by high purity methanol. The separation was performed on a WondaSil Cls WR column （250 mm× 4.6 mm,5 μm）;with methanol and 0. 1% H3 PO4 solution as mobile phase. The flow rate was 1.0 mL/min, the detection wavelength was 270 and 327 nm, and the column temperature was 40 %. Results showed that, the linearity ranges within 4.24 -42.4 ng for chlorogenic acid,6.76 -67.6 ng for lutin,4. 16 -41.6 ng for baicalin,4. 90 -49. 0 ng for paeonol,4.34 - 43.4 ng for baicalein,4.28 - 42.8 ng for emodin, and 5.72 - 57.2 ng for chrysophanol and all correlation coefficients are higher than 0. 999. The average spiked recovery rates are from 92. 4% to 107.8% ,and the relative standard deviations （RSD） are from 3.81% to 7.38%. The method is simple, accurate and suitable for the quality control for the preparation of such blend plant extracts.
出处
《日用化学工业》
CAS
CSCD
北大核心
2016年第9期544-548,共5页
China Surfactant Detergent & Cosmetics
