摘要
目的 :探讨受体相互作用蛋白激酶4(receptor interacting protein kinase 4,RIPK4)、β-catenin和P-糖蛋白(P-glycoprotein,P-gp)在骨肉瘤组织中的表达及其临床意义。方法:应用免疫组织化学法检测36例骨肉瘤和15例骨软骨瘤组织中RIPK4、β-catenin和P-gp的表达情况,分析三者的表达与骨肉瘤患者临床病理特征及预后之间的关系。结果:RIPK4、β-catenin和P-gp在骨肉瘤组织中的阳性表达率分别为63.9%、75.0%和61.1%,均高于这3个蛋白在骨软骨瘤组织中的阳性表达率(6.7%、20.0%和0.0%)(P值均<0.05)。RIPK4的表达与肿瘤大小以及Enneking分期相关(P值均<0.05);β-catenin的表达与Enneking分期相关(P<0.05);P-gp的表达与Enneking分期和转移相关(P值均<0.05)。RIPK4与β-catenin(γ=0.634,P<0.01)、β-catenin与P-gp(γ=0.461,P<0.01)、RIPK4与P-gp(γ=0.468,P<0.01)在骨肉瘤组织中的表达均呈正相关。Kaplan-Meier生存分析发现,RIPK4、β-catenin和P-gp阳性表达组患者的生存时间均短于阴性表达组(P值均<0.05)。单因素COX回归分析发现,年龄、Enneking分期、转移、RIPK4和P-gp蛋白的阳性表达与患者总生存时间相关(P值均<0.05);多因素COX回归分析证实,年龄、转移和P-gp蛋白的阳性表达可作为骨肉瘤患者独立的预后因素子(P值均<0.05)。结论:RIPK4、β-catenin和P-gp在骨肉瘤组织中高表达,且与骨肉瘤的病理特征相关;三者在骨肉瘤发生和发展的过程中起着重要作用,并能影响患者的预后。
Objective： To investigate the expressions of receptor interacting protein kinase 4 （RIPK4）, β-catenin and P-glycoprotein （P-gp） in osteosarcoma tissues and their clinical significance Methods： The expressions of RIPK4, β-catenin and P-gp in 36 specimens of osteosarcoma tissues and 15 specimens of osteochondroma tissues were detected by immunohistochemistry. The correlations of RIPK4, β-catenin and P-gp expressions with clinical characteristics and prognosis of the patients with osteosarcoma were analyzed. Results： The positive expression rates of RIPK4, β-catenin and P-gp in osteosarcoma tissues were 63.9%, 75.0% and 61.1%, respectively, which were higher than those in osteochondroma tissues （6.7%, 20.0% and 0.0%, respectively） （all P 〈 0.05）. The expression of RIPK4 was associated with tumor size and Enneking stage of osteosarcoma （both P 〈 0.05）. The expression of β-catenin was associated with Enneking stage of osteosarcoma （P 〈 0.05）. The expression of P-gp was associated with Enneking stage and metastasis of osteosarcoma （both P 〈 0.05）. There were positive correlation between RIPK4 and 13-catenin （y = 0.634, P 〈 0.01）, β-catenin and P-gp （y = 0.461, P 〈 0.01）, and RIPK4 and P-gp （~＇ = 0.468, P 〈 0.01） in osteosarcoma tissues. Kaplan-Meier analysis showed that the survival time of patients with RIPK4-, β-cateninor P-gp-positive expression was shorter than that of the negative expression group （all P 〈 0.05）. Univariate analysis of COX proportional hazards regression model revealed that age, Enneking stage, metastasis, RIPK4- and P-gp-positive expressions were relevant to the overall survival of patients with osteosarcoma （all P 〈 0.05）. Multivariate analysis of COX proportional hazards regression model confirmed that age, metastasis and P-gp-positive expression could be as independent prognostic factors of osteosarcoma patients （all P 〈 0.05）. Conclusion： The expressions of RIPK4, β-catenin and P-gp are high in osteosarcoma tissues and being related to the clinical characteristics of osteosarcoma. The three proteins may play important roles in the development and progression of osteosarcoma, and they can affect the prognosis of osteosarcoma patients.
出处
《肿瘤》
CAS
CSCD
北大核心
2016年第9期1006-1012,共7页
Tumor
基金
甘肃省基础研究创新群体项目(编号:1308RJIA004)
