摘要
[目的]:研究苍菊清肝降脂方对脂肪肝大鼠肝组织脂肪沉积、氧化应激和脂质过氧化的影响。[方法]采用雄性Wistar大鼠予以CCl4联合高脂低蛋白饮食复制脂肪肝模型,大鼠随机分成正常组、模型组和中药干预组。实验结束处死大鼠,肝组织行油红O染色,并检测肝组织甘油三酯、SOD、GSH和MDA等指标。[结果]油红O染色发现中药干预组肝细胞脂肪变性明显减轻,尤其以大剂量组减轻程度更为明显,同时大鼠肝组织中TG含量较模型组均显著下降(小剂量组P<0.05,大剂量组P<0.01),大剂量组降低程度显著优于小剂量组(大剂量组与小剂量组比较P<0.05)。大鼠肝组织中SOD活性和GSH含量较模型组均明显升高,MDA含量则显著下降(小剂量组P<0.05,大剂量组P<0.01);大剂量组对SOD活力和MDA含量的改善程度显著优于小剂量组(大剂量组与小剂量组比较P<0.05),但大剂量组和小剂量组GSH含量差异无统计学意义(P>0.05)。[结论]苍菊清肝降脂方显著降低脂肪肝大鼠肝组织脂肪沉积,并能显著改善氧化应激和脂质过氧化的作用。
[Objective]To study the effect of CangjuQingganJiangzhi decoction on fat deposition,oxidative stress and lipid peroxidation in rats with fatty liver.[Methods]Male Wistar rats were used to reproduce fatty liver model by combining carbon tetrachloride and high fat and low protein diet.Rats were randomly divided into control group,model group and CangjuQingganJiangzhi decoctiongroups.At the end of the experiment,all rats were sacrificed and liver tissues were stained with oil red O,and the indexes of triglyceride（TG）,SOD,GSH and MDA in liver tissues were detected.[Results]Oil red O staining results indicated that fatty degeneration of liver cells was significantly reduced in intervention groups compared with model group,especially in high-dose group the degree was reduced more obviously.The content of TG was significantly decreased（low-dose groupP〈0.05,high-dose groupP〈0.01）and high-dose group decreased significantly better than that of low-dose group（high-dose group and low-dose groupP〈0.05）.In rat liver tissue compared with model group,SOD activity and GSH content were significantly increased,MDA content was significantly decreased（low-dose groupP〈0.05,high-dose groupP〈0.01）;SOD activity and MDA contentin high-dose group improved significantly better than low-dose group（high-dose group and low-dose groupP〈0.05）,but GSH content in high-dose group and low-dose group was insignificantly different（P〉0.05）.[Conclusion]CangjuQingganJiangzhi decoction can significantly decrease fat deposition,improve oxidative stress and lipid peroxidation in rat fatty liver.
出处
《中国中西医结合消化杂志》
CAS
2016年第8期578-581,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
上海市浦东新区卫计委基金(No:PW2014A-59)
上海市浦东新区科委基金(No:PKJ2014-Y37)
上海市中医药三年行动计划(No:ZY3-RCPY-1-1001
ZY3-JSFC-1-1011)
关键词
苍菊清肝降脂方
脂肪肝
甘油三酯
氧化应激
脂质过氧化
大鼠
Cangju Qinggan Jiangzhi decoction
fatty liver
triglyceride
oxidative stress
lipid peroxidation
rats
