摘要
为了豌豆遗传转化及优良品种快速繁殖提供借鉴,进行了豌豆组织培养及植株再生条件试验。结果表明:豌豆的叶柄是诱导愈伤组织的最佳材料,在MS培养基中添加2,4-D(1 mg/L)和BA(0.5mg/L),愈伤诱导率达100%,但愈伤组织在分化培养基中未能分化出芽;豌豆子叶节是芽分化的最佳材料,在分化培养基上可分化出丛生芽,不同激素条件下萌发率均达100%。在MS+ZT(1mg/L)条件下,丛生芽最长;而在MS+BA(10 mg/L)条件下,丛生芽最多。豌豆子叶节丛生芽在MS+IBA(0.5mg/L)培养基上生根率最高,达40%以上,试管苗的移栽成活率为90%以上。
In order to provide references for pea genetic transformation and the rapid propagation of fine varieties, the experiment of tissue culture and plant regeneration of pea was carried out. Results： Pea petiole is the ideal inducing material for callus, 2,4-D （1 mg/L） and BA （0.5 mg/L） were added into MS medium, and the callus induction rate reached 100%, but there were no buds on differentiation medium. Pea cotyledon was the best differentiation material, the germination rate of the cluster buds was up to 100% using different hormones. Under the condition of MS medium with ZT （1 mg/L）, the cluster buds were the longest, while the number of buds was the most on the MS medium with BA （1 mg/L）. The rooting rate of cluster buds was the highest on the MS medium with IBA （0.5 mg/L）, which could reach more than 40 %. The surviving ratio of transplanted plants could exceed 90 %.
出处
《贵州农业科学》
CAS
2016年第7期6-8,共3页
Guizhou Agricultural Sciences
基金
辽宁省自然科学基金"肌动蛋白异型体PEAC3在干旱胁迫中的作用及其响应机制"(2013020072)
关键词
豌豆
组织培养
植株再生
pea
tissue culture
plant regeneration
