摘要
在水温17～18℃和盐度30条件下,将初始体质量为（4.09±0.26）g的仿刺参饲养在15个循环水玻璃缸（容积100L）中,投喂在基础饲料中添加0%、3%、6%、9%和12%浒苔的饲料,于投喂后第7、14、28d和42d分别检测仿刺参的生长指标、消化酶（蛋白酶、淀粉酶、纤维素酶和褐藻酸酶）和体腔液免疫酶（溶菌酶、酸性磷酸酶、碱性磷酸酶和超氧化物歧化酶）的活性。试验结果表明,投喂试验饲料后第14、28d和42d：（1）饲料中添加6%和9%浒苔组仿刺参的特定生长率显著增加（P〈0.05）,在不同取样时间其他添加组仿刺参的的特定生长率与对照组差异不显著（P〉0.05）;（2）饲料中浒苔添加量为6%和9%试验组仿刺参的4种消化酶比活力显著高于对照组（P〈0.05）,其中6%组仿刺参的消化酶比活力最高;（3）饲料中浒苔添加量为6%时,仿刺参溶菌酶活力显著高于对照组及其他试验组（P〈0.05）;饲料中浒苔添加量为6%和9%时,仿刺参碱性磷酸酶活力和超氧化物歧化酶活力显著高于对照组及其他试验组（P〈0.05）;添加浒苔可以显著提高酸性磷酸酶活力,至第42d,试验组酸性磷酸酶活力高于对照组（P〈0.05）,且浒苔添加量为6%时活力最高。在本试验条件下,饲料中添加浒苔可以提高仿刺参的特定生长率、消化酶活力及免疫力,浒苔的最适添加量为6%～9%。
A 6-week feeding experiment was conducted to evaluate the effects of dietary green laver Enteromopha prolifera on growth,activities of digestive enzymes and immunity in sea cucumber Apostichopus japonicus with initial body weight of（4.09±0.26）g.The sea cucumber juveniles were reared in 15 tanks of each 100 Lin volume with recycling water and fed the diets containing 0%,3%,6%,9% and 12% of green laver powder at water temperature of 17—18 ℃ and a salinity of 30.The specific growth rate,and activities of digestive enzymes including protease,amylase,cellulase,and alginase and immune enzymes including lysozyme,acid phosphatase,alkaline phosphatase and superoxide dismutase were analyzed 7,14,28 and 42days after the sea cucumber were fed different diets.The results showed that there were significantly increase in specific growth rate in the sea cucumber fed the diets supplemented with 6% and 9%green laver on the days 14,28 and 42（P〈0.05）,without significant differences among other groups compared with control group（P〉0.05）.Compared with the control group,there were significantly higher activities of the digestive enzymes in the sea cucumber fed the diets containing 6%and 9% of green laver（P〈0.05）,the maximum in the sea cucumber fed the diets containing 6%of green laver（P〈0.05）.The sea cucumber fed the diet supplemented with 6% of green laver had significantly higher activities of lysozyme,acid phosphatase and superoxide dismutase than the juveniles in other groups（P〈0.05）.It is concluded that dietary green laver leads to significant increase in specific growth,activities of digestive enzymes and immunity of sea cucumber,with the optimal supplementation of 6%—9% under the present experimental conditions.
出处
《水产科学》
CAS
CSCD
北大核心
2016年第5期498-503,共6页
Fisheries Science
基金
青岛市关键技术攻关计划项目(12-6-1-7-hy)
关键词
仿刺参
饲料
浒苔
生长
消化酶
免疫力
Apostichopus japonicus
diet
Enteromorpha prolifera
growth
digestive enzyme
immunity
