摘要
将鲜芦荟去皮研磨、过滤,先用磷酸盐缓冲液(PBS)进行提取、稀释,再配制含不同质量浓度D-葡萄糖的线虫生长培养基(NGM)喂食培养线虫.以线虫寿命及运动能力等生理指标确定脂肪堆积模型的最佳糖诱导质量浓度;通过油红O染色法及Image J软件分析评估芦荟提取物的降脂作用.实验结果表明:100mL NGM中含2g D-葡萄糖为最佳糖诱导质量浓度,可用于构建线虫脂肪堆积模型的脂肪评价;芦荟提取物对线虫脂肪堆积模型有良好的降脂作用.
We peeled fresh Aloe chinensis Baker, extracted and diluted with PBS buffer. The nematodes were cultured on nematode growth medium containing with different mass concentrations of D-glucose, which was used to evaluate the optimal mass concentration by conventional physiological index such as lifespan, athletic ability of nematodes. And the effect of aloe extract on reducing fat accumulation was showed by oil red O staining and Image J software analysis. Experimental results show that the 20/oo （2 gD-glu/100 mL NGM） is the optimal induced mass concentration to establish the Caenorhabditis elegans fat accumulation model, and aloe extract has excellent effect on reducing fat accumulation model of C. elegans.
出处
《吉林大学学报（理学版）》
CAS
CSCD
北大核心
2016年第5期1181-1185,共5页
Journal of Jilin University:Science Edition
基金
吉林省自然科学基金(批准号:201015171)
吉林省医药产业发展专项基金(批准号:YYZX201150-2)
吉林省科技发展计划项目(批准号:201205017)
吉林大学大学生创新基金(批准号:2014A34116)
关键词
秀丽隐杆线虫
糖诱导
脂肪堆积
中国芦荟提取物
Caenorhabditis elegans
induced by D-galactose
fat accumulation
Aloe chinensis Baker extract
