摘要
从甘肃河西走廊荒漠地区盐碱土壤中分离出143株链霉菌疑似菌株,拮抗试验表明,其中112株菌对枯草芽孢杆菌(B.subtilis)、金黄色葡萄球菌(S.aureus)、大肠杆菌(E.coli)有拮抗作用;8株对E.coli BTH 101具有拮抗作用;33株对辣椒晚疫病菌(P.capsici)、甘蓝枯萎菌(F.oxysporum sp.conglutinans)及番茄灰霉菌(B.cinerea)有拮抗作用.16SrRNA基因序列分析表明,其中42株菌为链霉菌属菌种.实验表明:河西走廊荒漠土壤中存在着丰富的拮抗链霉菌资源.
Streptomyces strains and their antimicrobial activity,which are isolated from the soils of reed rhizosphere and nonrhizosphere in the middle of Hexi corridor,are reported. A total of 143 streptomyces strains are obtained in this study. Of these,112 strains strongly inhibit the growth of bacterial model strains B. subtilis, S. aureus and E. coli, and 8 strains strongly inhibit the growth of E. coli BTH 101. And 38 strains have a high antifungal activity for fungal model strains of P. capsici,F, oxysporum sp. conglutinans and B. cinerea. The similarity alignment of 16S rRNA gene sequences shows that all the antimicrobial strains obtained in this experiment have over 98% similarity with at least one known strain of streptomyces in the database. The re- suits show that there are abundant antagonistic streptomyces resources from the desert soil in Hexi corridor,and it is a foundation to develop new antibiotics.
出处
《兰州交通大学学报》
CAS
2016年第4期127-133,共7页
Journal of Lanzhou Jiaotong University
基金
国家国际科技合作专项(2011DFA32520)
国家自然科学基金(31260090)
关键词
链霉菌
抗菌活性
固态发酵
streptomyces
antimicrobial activity
solid-state fermentation
