摘要
目的探讨切除修复交叉互补基因1（ERCC1）-4533/8092位点单核苷酸多态性（SNPs）与广西壮族人群肝癌易感性之间关系。方法通过聚合酶链反应限制性片段长度多态性（PCR-RFLP）方法检测88例原发性肝癌患者和82例健康对照者的ERCC1-4533/8092基因多态性。结果 ERCC1-4533位点的基因分型在病例组和对照组的频数分布差异无统计学意义（P〉0.05）,ERCC1-8092位点的基因分型在病例组和对照组的频数分布差异具有统计学意义（P〈0.05）。与携带ERCC1-8092CC基因型的个体相比,携带ERCC1-C8092CA/AA基因型的个体具有更高的肝癌易感性（CA：OR=2.556,95%CI：1.345~4.855;AA：OR=8.667,95%CI：1.000~75.092）。以携带ERCC1-8092C等位基因作为参照,携带ERCC1-C8092A等位基因可以增加原发性肝癌的发病危险性（OR=2.387,95%CI：1.428~3.992）。结论 ERCC1-8092位点基因多态性与广西壮族人群肝癌易感性有关。
Objective To investigate the relationship on the excision repair cross complementing gene 1（ERCC1）-4533/8092 site single nucleotide polymorphisms（SNPs）and the susceptibility to hepatocellular carcinoma（HCC）in Guangxi Zhuang population.Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）method was used to detect the ERCC1-4533/8092 gene polymorphism in 88 cases with primary liver cancer and 82 cases of normal controls.Results There was no difference in the frequency distribution of ERCC1-4533 in the case group and the control group,the frequency distribution of the ERCC1-8092 in the case group and the control group was different（P〈0.05）.Compared with ERCC1-8092 CC,ERCC1-C8092CA/AA had higher risk of primary hepatocellular carcinoma（CA：OR=2.556,95%CI：1.345-4.855;AA：OR=8.667,95%CI：1.000-75.092）.ERCC1-8092 Callele as a reference,ERCC1-8092 Aallele can increase the risk of primary liver cancer（OR=2.387,95%CI：1.428-3.992）.Conclusion The genetic polymorphisms of ERCC1-8092 sites are associated with susceptibility to hepatocellular carcinoma in Guangxi Zhuang population.
出处
《国际检验医学杂志》
CAS
2016年第18期2523-2525,共3页
International Journal of Laboratory Medicine
基金
广西自然科学基金资助项目(2012GXNSFAA053170)
关键词
ERCC1
原发性肝癌
基因多态性
易感性
ERCC1
primary hepatocellular carcinoma
polymorphisms
susceptibility
