摘要
目的:探讨大柴胡汤加减方对肝功能和凝血机制的作用。方法:50只大鼠随机分为3组,正常对照组(A组)10只,模型组(B组)20只,大柴胡汤组(C组)20只。C组大鼠于造模前2 d分别灌胃大柴胡汤10 mL/(kg·d),A、B组予同等剂量0.9%氯化钠注射液灌胃,B、C组大鼠均采用皮下注射硫代乙酰胺0.2 g/kg,24 h后相同剂量重复皮注1次,48 h后完成急性肝衰竭动物模型,首次注射硫代乙酰胺的大鼠,予皮下注射10%葡萄糖注射液0.25 g/kg。观察造模完成后继续予大柴胡汤灌胃3 d,A组大鼠予同等剂量0.9%氯化钠注射液灌胃,3 d后取血处死所有大鼠,保存大鼠血清和病理组织。观察3组大鼠病理组织、肝功能(ALT、AST、ALB、CHE)、凝血功能(PT)指标的情况。结果:与模型组比较,大柴胡汤组大鼠组织肝衰竭减轻;模型组大鼠ALT、AST、TBiL与正常组大鼠比较,明显升高,差异具有统计学意义(P<0.05),模型组大鼠ALB、CHE与正常组大鼠比较,显著降低,差异具有统计学意义(P<0.05),大柴胡汤组大鼠ALT、AST、TBiL与模型组大鼠比较,明显降低,差异具有统计学意义(P<0.05),大柴胡汤组大鼠ALB、CHE与正常组大鼠比较,显著升高,差异具有统计学意义(P<0.05);模型组大鼠PT与正常组大鼠比较,明显升高,差异具有统计学意义(P<0.05),大柴胡汤组大鼠PT与模型组大鼠比较,明显降低,差异具有统计学意义(P<0.05)。结论:大柴胡汤加减方对肝功能和凝血机制具有保护作用。
Objective：To evaluate the Modified Major Bupleurum Decoctions effect on liver function and blood coagulation mecha-nism.Methods：A total of 50 rats were randomly divided into three groups：normal group （A group）with 10 rats,model group （B group）with 20 rats and Bupleurum Decoction group （C group）with 20 rats.C group were given 10 mL/（kg.d）oral Bupleurum Decoction 2 days before modeling.A and B group were given the same dose of 0.9%NaCl.B and C group rats were given subcuta-neous injection of thioacetamide 0.2 g/kg per 24h for two days and then the acute liver failure models were established.For rats in-jected with thioacetamide for the first time,10%GS 0.25 g/kg were also administrated.After the model was established,Major Bup-leurum Decoction lavage were given for three days,and A group rats were given the same dose of 0.9%NaCl.Three days later,all the rats were killed to take blood sample,and rats serum and tissue pathology were kept after 3 days.Pathological tissue,liver func-tion indexes （ALT,AST,ALB,CHE）,blood coagulation function indexes （PT）were compared among the three groups.Results：Compared with model group,the tissue of Major Bupleurum Decoction group was better;ALT,AST,TBiL of model group leveled up significantly compared with normal group rats（P 〈0.05）,ALB and CHE of model group rats decreased significantly compared with normal group （P 〈0.05）.Compared with model group,ALT,AST,TBiL of Major Bupleurum Decoction group decreased signifi-cantly（P 〈0.05）,ALB、CHE of Major Bupleurum Decoction group increased significantly （P 〈0.05）;PT of model group in-creased significantly compared with the normal group（P 〈0.05）,PT of Major Bupleurum Decoction group decreased significantly compared with model group（P 〈0.05）.Conclusion：Modified Major Bupleurum Decoction plays a protective role in liver function and blood coagulation mechanism.
作者
汉利
王新伟
单恒云
张晓
Han li Wang Xinwei Shan Hengyun Zhang Xiao(Rizhao City Hospital of traditional Chinese Medicine, Rizhao 276500, Chin)
出处
《世界中医药》
CAS
2016年第6期1063-1065,1069,共4页
World Chinese Medicine
关键词
大柴胡汤加减
肝功能
凝血机制
急性肝衰竭
研究
Major Bupleurum Decoction
Liver function
Blood coagulation mechanism
Acute hepatic failure
Research
