摘要
为比较2种血清型绿脓杆菌生长特性的差异,采用分光光度法和活菌计数法测定绿脓杆菌血清B型分离株SD004株和血清C型分离株WD013株的生长曲线,对两种方法绘制的生长曲线进行比较。结果表明,两种方法绘制曲线有差异,对数生长期和稳定期菌液OD600nm值与活菌计数对数之间呈现一元二次多项式回归关系。该试验为貂源绿脓杆菌生物学特性的比较研究及疫苗制备提供参考。
To compare the growth characteristics between two serotypes of Pseudomonas aeruginpsa, growth curves of both isolates was detected by spectrophptpmetry method and colony count method. The results showed that two curves were different for the two methods. The relationships between OD600nm value and Log10 CFU/mL was quadratic polynomial with one variable at logarithmic phase and stationary phase. The results of the study was pre- pared for biological characteristics and vaccine of P. aeruginpsa from the Minks.
作者
杨朋欣
丁国杰
刘可姝
张春媛
王彬
YANG Pengxin DING Guojie LIU Keshu ZHANG Chunyuan WANG Bin(Harbin Pharmaceutical Group Bio-vaecine Co., Ltd., Harbin 150069, China)
出处
《广东畜牧兽医科技》
2016年第4期41-43,共3页
Guangdong Journal of Animal and Veterinary Science
关键词
绿脓杆菌
血清型
生长曲线
生长特性
Pseudomonas aeruginpsa
serotype
growth curve
growth characteristic
