摘要
目的研究羟基红花黄色素A(HSYA)对小鼠Lewis肺癌移植瘤中微血管密度(MVD)及血管内皮生长因子(VEGF)mRNA表达的影响,探讨HSYA抑制肿瘤的分子机制。方法运用C57/BL小鼠接种Lewis肺癌细胞株于右侧肋腹皮下建立小鼠肺癌移植瘤模型,随机分为对照组(生理盐水)、环磷酰胺组(CTX)、HSYA不同剂量组(28 mg/L、56 mg/L、112 mg/L),给药方式均为腹腔注射给药。用药22 d后脱颈处死小鼠并剥离肿瘤组织,采用免疫组织化学法检测MVD,采用实时荧光定量PCR检测各组肿瘤组织中VEGF mRNA的表达情况。结果 56 mg/L、28 mg/L HSYA组与CTX组小鼠肿瘤组织MVD分别为30.01±3.12、22.56±2.11、16.21±2.40,显著低于对照组的41.10±2.93以及112 mg/L HSYA组的37.66±3.04,差异有统计学意义(X^2=2.82,P=0.010;X^2=3.16,P=0.007;X^2=4.58,P=0.000)及(X^2=1.98,P=0.038;X^2=2.45,P=0.016;X^2=3.82,P=0.001)。28 mg/L HSYA组与对照组VEGF扩增荧光表达阈值所需循环次数比较,差异无统计学意义,但其VEGF mRNA的表达值仅为0.43±0.16,明显低于对照组的0.82±0.06,差异有统计学意义(F=0.77,P=0.038)。结论 28 mg/L HSYA能够使小鼠Lewis肺癌移植中MVD明显减少,具有抑制肿瘤生长的作用,可能与下调VEGF mRNA表达有关。
Objective To observe the effects of hydroxysafflor yellow A （HSYA） on microvessel density （MVD） of mice transplanted Lewis lung cancer and mRNA expression of vascular endothelial growth factor （VEGF） so as to explore the tumor-inhibiting mechanism of HSYA. Methods Sixty tumor-bearing C57/BL mice were randomly divided into five groups, with 12 mice in each group, namely a control group, a cyclophosphamide （CTX） group （25mg/kg）, a large dose HSYA group （112mg/L）, a medium dose HSYA group （56mg/L） ,and a small dose HSYA group （28mg/L）. These different drugs were administered by intraperitoneal injection. The mice were sacrificed 22 days after the treatment. Tumor tissues were sampled and examined by immunohistochemical method and quantitative real-time PCR to detect the expression of MVD and VEGF mRNA. Results The MVD of the medium and small dose HSYA groups and CTX group were 30. 01 ±3.12,22. 56 ：t：2. 11 and 16. 21 ±2.40,respectively,which were significantly lower than 41.10 ± 2. 93 of the control group and 37. 66 ± 3.04 of the large dose HSYA group （ χ2 = 2. 82, P = 0. 010;χ2 =3. 16,P =0. 007;χ2 =4. 58,P =0. 000） and （χ2 = 1.98,χ2 =0.038;χ2 =2.45,P =0. 016; χ2 = 3.82, P = 0. 001 ）. The difference in VEGF amplified fluorescence expression threshold between theHSYA groups and the control group was not significant. However, after amplification, the expression of VEGF mRNA in the small dose HSYA group was only 0. 43 ±0. 16,which was obviously lower than 0. 82 ±0.06 in the control group （ F = 0. 77, P = 0. 038 ）. Conclusion HSYA can significantly reduce MVD in mice transplanted Lewis lung cancer and down-regulate expression of VEGF mRNA to achieve tumor-inhibiting effect.
出处
《中国呼吸与危重监护杂志》
CAS
北大核心
2016年第5期461-464,共4页
Chinese Journal of Respiratory and Critical Care Medicine
基金
陕西省教育厅科学研究项目(编号:14JK1208)
咸阳市中心医院重点培育项目
关键词
羟基红花黄色素A
Lewis肺癌移植瘤
微血管密度
血管内皮生长因子
Hydroxysafflor yellow A
Transplanted tumor of Lewis lung cancer
Microvesseldensity
Vascular endothelial growth factor
