摘要
黄曲霉毒素是黄曲霉菌和寄生曲霉菌分泌的次级代谢产物,容易污染粮食作物及其制品,主要存在于发霉的粮食、豆类、坚果及与其相关食品中。黄曲霉毒素,具有极强的毒性和致癌性,尤其是黄曲霉毒素B1,是目前已发现的最强的天然致癌物质,严重威胁人类健康。目前食品中黄曲霉毒素的检测以高效液相色谱、液质联用等仪器分析方法为主,然而仪器分析方法具有设备昂贵、操作繁琐、需要专业人员等不足。近年来以特异性生物分子(抗体、重组抗体、适配体等)识别黄曲霉毒素,并结合不同的信号报告分子,建立了一系列黄曲霉毒素分析方法,检测快速、灵敏并易于操作,在食品安全领域广泛应用。着重从黄曲霉毒素的生物识别分子和信号报告分子两方面介绍目前黄曲霉毒素的快速检测手段,并对其进行比较和评价,同时对黄曲霉毒素快速检测方法的发展方向进行展望。
Aflatoxins are secondary metabolites secreted by Aspergillus flavus and Aspergillus parasiticus,easily pollute the grains and their processed products,and mainly exist in moldy grain,legumes,nuts and associated food products. Aflatoxins are extremely toxic and carcinogenic,especially aflatoxin B1 that has been found to be the strongest natural carcinogen and severely threat to human health. Current detection methods of aflatoxins in the grains are mainly instrumental analysis by combining the high performance liquid chromatography and liquid mass spectrometry,however,instrumental analysis presents the shortcomings such as instruments cost is very high,analysis process is very complex and needs long-time-trained professionals. In recent years,integrating the rapid identification of aflatoxin based on biological binders（antibody,recombinant antibody,and aptamer）and different signal reporters,a series of analysis methods for aflatoxin were established,and they are fast,flexible,and easy to operate,therefore widely applied in food safety. Here we review the rapid detection methods mainly from 2 aspects of specific biological binders for aflatoxins and signal reporters,compare and evaluate them,also prospect the development trend of rapid methods for aflatoxin.
作者
曾昆
杜道林
薛永来
ZENG Kun DU Dao-lin XUE Yong-lai(College of Envirottmental and Safety Engineering, Institute of Environment and Ecology, Jiangsu University, Zhenjiang 212013)
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第8期47-55,共9页
Biotechnology Bulletin
基金
国家自然科学基金项目(31502118
31170386
31570414)
江苏省自然科学基金项目(BK20130507
BK2012274)
中国博士后基金项目(2013M541606)
江苏省农业科技自主创新资金[CX(13)3093]
江苏大学高级人才启动金(13JDG016)
镇江市科技支撑计划项目(NY2013006)
镇江市国际科技合作项目(GJ2014008)
