摘要
为了解天津地区猪繁殖与呼吸综合征病毒(PRRSV)流行毒株的遗传变异情况和流行趋势,用RT-PCR对2014年—2015年在天津地区采集的疑似PRRS病料检测,并扩增阳性病料PRRSV NPS2部分基因片段和进行PRRSV分离鉴定。结果显示,237份疑似PRRSV感染病料中64份为阳性,阳性率为27%,分离获得33株PRRSV;Nsp2序列分析发现,天津地区流行的PRRSV主要为美洲型,其中54份与高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)高度同源,8份与经典PRRSV高度同源,2份与NADC30-like高度同源。结果表明,2014年—2015年天津地区主要流行HP-PRRSV,并出现了NADC30-like变异毒株,说明PRRSV流行种类的多样化,提示加强PRRSV流行及变异监测十分必要。
This study aimed at analyzing the genetic variation and the development trend of PRRSV in Tian- jin from 2014 to 2015. 237clinical samples collected from 2014 to 2015 in different districts of Tianjin were tested by RT-PCR to amplify partial Nsp2 and isolate virus using positive samples. The result showed that 64 samples were positive, the positive rate was 27%, and acquired 33 PRRSV isolates were field; partial Nsp2 gene and phylogenetic analysis revealed that most of pandemic strains were the North American prototype,and 54 samples were highly homologous with the highly pathogenic PRRSV（HP-PRRSV）,8 samples were highly homologous with classic PRRSV, 2 samples were highly homologous with North American pandemic strain NADC30. The study indicated that HP-PRRSV was the popular strain in Tianjin during 2014-2015, the NADC30-like PRRSV emerged make the category of PRRSV variant more diverse,pointing that it is important to pay more attention to monitor prevalent and genetic variation of PRRSV.
出处
《动物医学进展》
北大核心
2016年第10期11-15,共5页
Progress In Veterinary Medicine
关键词
猪繁殖与呼吸综合征病毒
分离
分子流行病学
遗传演化分析
Porcine reproductive and respiratory syndrome virus
isolation
molecular epidemiology
phylo- genetic analysis
