期刊文献+

萝卜-芥蓝异源四倍体植物SR30基因转录本的选择性剪接分析

Alternative Splicing Analysis of SR30 Isoforms in Rapahnobrassica Allotraploid Plant
下载PDF
导出
摘要 为了解异源多倍体形成后,其剪接因子基因SR30在各组织器官间的表达量以及选择性剪接模式与亲本的差异,选取萝卜-芥蓝异源四倍体(Raphanobrassica)及其亲本萝卜(Raphanus sativus)、芥蓝(Brassica oleracea var.alboglabra)为材料,运用RACE-PCR方法克隆到全长的编码序列(CDS)和3非编码区(3 UTR),运用q RT-PCR和半定量RT-PCR检测其在各组织器官中的表达量和各转录本表达量间的差异。结果表明,四倍体中萝卜同源的Rs SR30基因有5种转录本,芥蓝同源的Bo SR30基因有4种转录本。同时,SR30在3物种中的表达具有组织器官的差异,且在四倍体中的总体表达量显著低于亲本。根据克隆到的转录本,预测Rs SR30编码3种蛋白,Bo SR30编码2种,不同蛋白异构体的区别体现在C末端的丝氨酸-精氨酸富集(RS)结构域。因此,萝卜-芥蓝异源多倍体形成后,SR30基因在表达量和转录本选择性剪接方面都发生了改变。 In order to understand the difference between allopolyploid and its parents in expression level and alternative splicing pattern of SR30 gene when the allopolyploid had been synthesized, different tissues or organs were collected from allotetraploid plant Raphanobrassica and its maternal parent Raphanus sativus, paternal parent Brassica oleracea var. alboglabra. Full-length coding sequence(CDS) and 3 untranslated regions(3 UTR) were cloned by using RACE-PCR. The expression level based on q RT-PCR and relative expression of each m RNA isoforms by using semi-quantitative RT-PCR was detected. The results showed that there were 5 m RNA isoforms of Rs SR30 and 4 m RNA isoforms of Bo SR30 appeared in the allopolyploid. In addition, the expression of SR30 reflected an obvious diversity between different tissues or organs. The expression of SR30 reduced significantly in allopolyploid compared with its parental species. Based on the m RNAs, it was predicted that there are 3 proteins encoded by Rs SR30 and 2 proteins encoded by Bo SR30. The difference between each protein isoforms was reflected in the RS(rich in serine-arginine dipeptides) domains located in C-terminal. Therefore, both of expression and alternative splicing patterns of SR30 changed when the allopolyploid had been synthesized.
出处 《热带亚热带植物学报》 CAS CSCD 北大核心 2016年第5期535-544,共10页 Journal of Tropical and Subtropical Botany
基金 国家自然科学基金项目(31370258)资助~~
关键词 异源多倍体 SR30基因 选择性剪接 基因表达 萝卜 芥蓝 Allopolyploid SR30 Alternative splicing Gene expression Raphanus sativus Brassica oleracea var.alboglabra
  • 相关文献

参考文献34

  • 1WOOD T E, TAKEBAYASHI N, BARKER M S, et al. The frequencyof polyploid speciation in vascular plants [J]. Proc Natl Acad Sci USA,2009, 106(33): 13875–13879. doi: 10.1073/pnas.0811575106.
  • 2JIAO Y N, WICKETT N J, AYYAMPALAYAM S, et al. Ancestralpolyploidy in seed plants and angiosperms [J]. Nature, 2011, 473(7345):97–100. doi: 10.1038/nature09916.
  • 3ADAMS K L, PERCIFIELD R, WENDEL J F. Organ-specificsilencing of duplicated genes in a newly synthesized cotton allotetraploid[J]. Genetics, 2004, 168(4): 2217–2226. doi: 10.1534/genetics.104.033522.
  • 4YOO M J, LIU X X, PIRES J C, et al. Nonadditive gene expression inpolyploids [J]. Annu Rev Genet, 2014, 48: 485–517. doi: 10.1146/annurev-genet-120213-092159.
  • 5YOO M J, SZADKOWSKI E, WENDEL J F. Homoeolog expressionbias and expression level dominance in allopolyploid cotton [J].Heredity, 2013, 110(2): 171–180. doi: 10.1038/hdy.2012.94.
  • 6FLAGEL L E, WENDEL J F. Evolutionary rate variation, genomicdominance and duplicate gene expression evolution during allotetraploidcotton speciation [J]. New Phytol, 2010, 186(1): 184–193. doi: 10.1111/j.1469-8137.2009.03107.x.
  • 7桂琴,徐延浩,王建波.多倍体植物中基因表达模式的变化[J].武汉植物学研究,2007,25(2):198-202. 被引量:5
  • 8李智慧,逯翔,高云,刘少军,陶敏,肖蘅,乔云倩,张亚平,罗静.物种多倍化与表观遗传学[J].科学通报,2010,55(35):3358-3366. 被引量:3
  • 9聂利红,王延召,房卫平,谢德意.多倍体植物基因组结构和基因表达的变化研究进展[J].河南农业科学,2012,41(2):1-4. 被引量:4
  • 10REDDY A S N, MARQUEZ Y, KALYNA M, et al. Complexity of thealternative splicing landscape in plants [J]. Plant Cell, 2013, 25(10):3657–3683. doi: 10.1105/tpc.113.117523.

二级参考文献266

共引文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部