摘要
目的建立丹参配方颗粒的HPLC指纹图谱。方法以丹酚酸B为参照物,利用高效液相色谱梯度洗脱,测定了11批丹参配方颗粒样品;色谱柱为Waters Sun Fire C18(4.6mm×250mm,5μm);流动相:甲醇(A)-0.4%的甲酸溶液(B),检测波长270nm,柱温30℃,流速1.0m L·min-1。结果通过HPLC指纹图谱分析,标示出丹参配方颗粒16个共有色谱峰,相似度均大于0.96,并确认2个已知峰为丹酚酸B和丹参酮ⅡA。结论建立的丹参配方颗粒的HPLC指纹图谱稳定可靠,操作简便,可为丹参配方颗粒质量控制提供科学依据。
OBJECTIVE To establish an HPLC method for determination of the fingerprint of Salvia Dispensing Granule. METHODS 11 samples were analyzed by HPLC, with Salvianolic acid B as the references. Analysis was performed on a Waters SunFire Cls column （4. 6mm × 250mm, 51μm） with mobile phase A： Methanol and mobile phase B ：0. 4% Formic acid solution by gradient elution;flow rate was 1.0mL ~ min-1, k = 270nm, Column box tem- perature was 30~C. RESULTS By analyzing the Chromatogram fingerprint, 16 peaks existed which included Salvi- anolic acid B and Tanshinone II A. The similarity of 11 samples was more than 0. 96. CONCLUSION The estab- lished HPLC fingerprint Chromatogram of Salvia Dispensing Granule methed is dependable and simple. This method can provide scientific basis for the quality control of Salvia dispensing Granule.
出处
《海峡药学》
2016年第9期45-49,共5页
Strait Pharmaceutical Journal
基金
2014年湖北省中青年创新团队项目(编号:T201414
武当山区道地中药材GAP关键技术研究)
关键词
丹参
配方颗粒
HPLC
指纹图谱
丹酚酸B
丹参酮ⅡA
Salvia
Dispensing Granule
HPLC
Chromatogram fingerprint
Salvianolic acid B
TanshinoneⅡA
