摘要
目的探讨不同黑色素瘤细胞株对盐霉素的敏感性,及盐霉素对敏感细胞株细胞周期的影响。方法 8种不同黑色素瘤细胞株,采用不同浓度盐霉素(40、20、10、5、1.25、0.625、0.312 5μmol/L)作用3d,计算盐霉素对8种黑色素瘤细胞株的半数抑制浓度(half maximal inhibitory concentration,IC50),挑选对盐霉素敏感的黑色素瘤细胞株;将敏感黑色素瘤细胞株分为对照组、0.1μmol/L盐霉素组、0.3μmol/L盐霉素组和1.0μmol/L盐霉素组,分别给予0、0.1、0.3、1.0μmol/L盐霉素处理细胞,处理48h时采用流式细胞仪观察各组细胞周期变化情况,1.0μmol/L盐霉素组处理24、48h后观察细胞形态的变化情况。结果 SK-MEL-19细胞对盐霉素的IC50[(0.82±0.60)μmol/L]最低,低于其他7种细胞株,SK-MEL-19细胞对盐霉素最敏感;作用48h后,1.0μmol/L盐霉素组SK-MEL-19细胞G0G1期[(39.90±3.43)%]、G2M期[(4.03±1.51)%]、S期[(33.99±1.09)%]细胞比例明显低于对照组[(48.92±1.14)%、(6.39±0.77)%、(44.69±1.36)%]、0.1μmol/L盐霉素组[(44.65±1.13)%、(8.23±0.96)%、(47.12±1.18)%]和0.3μmol/L盐霉素组[(46.18±2.88)%、(8.20±2.17)%、(45.62±1.83)%](P<0.05),对照组及0.1、0.3μmol/L盐霉素组细胞周期比例两两比较差异均无统计学意义(P>0.05);1.0μmol/L盐霉素组作用24h后部分SK-MEL-19细胞出现空泡,作用48h后细胞出现明显死亡,且细胞周期有小凋亡峰(subG1峰)出现,有细胞凋亡存在。结论盐霉素对SK-MEL-19黑色素瘤细胞株有较强杀伤作用,此杀伤作用可能是通过诱导细胞凋亡实现的。
Objective To explore the sensitivity of different melanoma cell lines to salinomycin and the influence of salinomycin on cell cycle in the most sensitive cell line. Methods Eight types of melanoma cell lines were treated by using different concentrations of salinomycin （40, 20, 10, 5, 1.25, 0. 625, 0. 312 5μmol/L） for 3 days. The most sensitive cell lines were chosen after the half maximal inhibitory concentration （IC50） values were calculated, and were divided into control group, 0.1 9mol/L salinomycin group, 0.3 9mol/L salinornycin group and 1.0μmol/L salinomycin group treated with 0, 0.1, 0.3 and 1.0μmol/L salinomycin, respectively. The changes of cell cycle were detected by flow cytometry after 48 h. The change of cell morphology was observed on microscope in 24 and 48 h in 1.0 μmol/L salinomycin group. Results The ICs0 of SK-MEL-19 to salinomycin （（0. 82±0.60）μmol/L） was significantly lower than that of other 7 kinds of cell lines, indicating that SK-Mel-19 was most sensitive to salinomycin treatment. After treated with salinomycin for 48 h, the cell proportions of GoG1 phase （（39. 90±3.43）0/00）, G2M phase （（4. 034±1.51）%） and S phase （（33. 99± 1.09） %） in 1.0 μmol/L salinomycin group were significantly lower than those in control group （（48. 92± 1.14） %, （6.39±0.77）%, （44.69±1. 36）%）, 0. 1μmol/L salinomyein group （（44.65±1.13）, （8.23±0.96）%, （47.12±1. 18）%） and 0.3 μmol/L salinomycin group （（46.18±2.88）%, （8.20±2.17）%, （45.62±1.83）%） （P〈0.05）, and there were no significant differences between every two groups of control group, 0. 1 μmol/L group and 0.3 μmol/L group （P〈0.05）. Some SK-MEL-19 cells appeared cavity after treated with 1. 0μmol/L salinomycin for 24 h, and obvious cell death and apoptosis peak （subG1 peak） were observed after treated with 1.0 μmol/L salinomycin for 48 h, which demonstrated the presence of apoptosis. Conclusion Salinomycin has a strong killing effect on SK-MEL-19, which may be achieved by inducing apoptosis.
出处
《中华实用诊断与治疗杂志》
2016年第10期962-964,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
广东省自然科学基金(2015A030313754)
