摘要
目的提升实验室对食品中金黄色葡萄球菌的检测能力。方法按照能力验证作业指导书规定的方法要求进行检测,并采用全自动微生物基因指纹鉴定系统(RiboPrinter^(?))对盲样中添加的菌株进行鉴定与分型。结果编号为CODE 1~9的样品均检出金黄色葡萄球菌,编号为CODE 10的样品未检出金黄色葡萄球菌。阳性样品的Z值结果均<2;阴性样品的结果为<10 CFU/g,10个样品测试均取得满意结果。结论通过开展能力验证工作可以较好地提高实验室检测能力。
Objective To improve the detection capacity of Staphylococcus aureus in food. Methods Detection was followed the instruction of the proficiency testing, and RiboPrinter was used for identification and ribotyping of the bacteria added into the blind sample. Results Staphylococcus aureus was detected in CODE 1-9 and there was no Staphylococcus aureus in CODE 10. The Z scores of the positive samples were all less than 2 and the result of the negative sample was less than 10 CFU/g. The test results of 10 samples were satisfied. Conclusion The laboratory detection capability has been improved by implementing proficiency testing.
作者
钟玮
张宁
刘玉萍
范一灵
杨美成
ZHONG Wei ZHANG Ning LIU Yu-Ping FAN Yi-Ling YANG Mei-Cheng(Shanghai Institute for Food and Drug Control, Shanghai 201203, China)
出处
《食品安全质量检测学报》
CAS
2016年第7期2758-2762,共5页
Journal of Food Safety and Quality
关键词
金黄色葡萄球菌
定量
能力验证
鉴定
分型
Staphylococcus aureus
quantitative
proficiency testing
identification
ribotyping
