摘要
在高等植物花药发育和花粉形成中,MYB转录因子起着非常重要的作用,其中MYB80是参与绒毡层发育及引起雄性不育的重要转录因子。该研究以拟南芥(Arabidopsis thaliana)At MYB80为参考序列,通过BLAST比对分析,在白菜(Brassica rapa)、甘蓝(B.oleracea)和甘蓝型油菜(B.napus)中分别获得MYB80基因的2、2和6个同源序列,运用生物信息学方法对其核苷酸序列及编码的氨基酸序列进行组成成分、亚细胞定位、磷酸化位点、疏水性/亲水性、蛋白质二级、三级结构和功能域分析。结果表明,MYB80转录因子亚细胞定位于细胞核,具有多个不同的磷酸化位点,肽链表现为亲水性;二级、三级结构预测显示,MYB80蛋白以α-螺旋和无规则卷曲为主要结构元件;保守结构域分析表明,其N端具有2个串联的SANT功能域,属于R2R3型MYB转录因子。多重序列比对和进化树分析结果表明,甘蓝型油菜与白菜、甘蓝的序列相似性大于92%,且MYB80转录因子的功能结构域具有较高的同源性和较强的序列保守性。该研究结果对深入解析甘蓝型油菜MYB80的生物学功能及育性调控的分子机理具有重要意义,为甘蓝型油菜杂种优势利用提供了依据。
MYB transcription factors play a key role in anther development and pollen formation in higher plants, and MYB80 is an important transcription factor involved in the development of tapetum and initiation of male sterility. In this study, we performed BLASTN and TBLASTX searches of the Arabidopsis thaliana MYB80 （AtMYBS0） amino acid se- quences against the published database and identified 2, 2 and 6 homologous sequences of MYB80 gene in Brassica rapa, B. oleracea and B. napus, respectively. The composition of nucleic acid sequence and amino acid sequences, sub- cellular localization, phosphorylation sites, hydrophilicity and hydrophobicity, secondary and tertiary structure of protein, conserved domains of the MYB80 were predicted and analyzed by bioinformatics tools and resources from the Internet. The results showed that the MYB80 transcription factors are located in the nucleus by analyzing subcellular localization, with a number of different phosphorylation sites, and the peptide chains are hydrophilia. The predicted secondary struc- ture and tertiary structure showed that the main structural elements of the MYB80 protein were a helix and random coil. Conserved domain analysis indicated that the MYB80 transcription factor belongs to the MYB-R2R3 type transcription factor, with 2 tandem SANT domains in the N-terminal sequence. The multiple sequence ratio and phylogenetic tree were constructed with MYB80 proteins from different plants; similarity among B. napus and B. rapa, and B. oleracea was more than 92%, and the conserved functional domains had high homology and strong sequence conservation. These results are helpful for resolving the biological functions of MYB80 and the molecular mechanism of sterility regulation and lay a foundation for the heterosis utilization in B. napus.
出处
《植物学报》
CAS
CSCD
北大核心
2016年第5期620-630,共11页
Chinese Bulletin of Botany
基金
国家重点研发计划"七大农作物育种"专项(No.2016YFD0101300)
国家自然科学基金(No.U1302266)
