摘要
目的建立测定护肝布祖热颗粒中秦皮乙素含量的超高效液相色谱（UHPLC）法。方法色谱柱为Acquity UPLC BEH C18柱（100 mm×2.1 mm,1.7μm）,流动相为乙腈-0.4%磷酸溶液梯度洗脱,流速为0.21 m L/min,柱温为30℃,检测波长为349 nm,进样量为1μL。结果秦皮乙素质量浓度在1.06-21.16μg/m L范围内与峰面积线性关系良好（r=0.999 7）;加样回收率为100.65%,RSD为0.97%（n=6）。结论该方法简便、准确,测得结果稳定性、重复性好,可用于该制剂中秦皮乙素含量测定。
Objective To establish an UHPLC method for the content determination method of aesculetin in Hugan Buzure Granules. Methods The column was Acquity UPLC BEH C18 column （ 100 mm × 2. 1 mm, 1. 7 μm ）;the mobile phase was acetonitrile -0. 4%phosphoric acid solution, gradient elution;the flow rate was 0. 21 mL/min, the column temperature was 30 ℃, the detection wavelength was 349 nm, the sample volume was 1 μL. Results Aesculetin showed good linear relationship in 1. 06-21. 16 μg/mL （ r=0. 999 7 ） , the average recovery rate was 100. 65% and the RSD=0. 97%（ n=6 ） . Conclusion The method is simple, accurate and reproducible. It can be used for the content determination of aesculetin in the preparation.
出处
《中国药业》
CAS
2016年第19期56-58,共3页
China Pharmaceuticals
