摘要
背景:建立安全可靠、容易重复的非酒精性脂肪肝小鼠模型是研究该病诊治方案的前提条件。目的:建立C57BL/6非酒精性脂肪性肝病小鼠模型并观察其生化指标的变化特点,为其发病机制及药物治疗提供理论依据。方法选用健康雄性C57BL/6小鼠60只,随机分组,对照组30只采用普通饲料喂养,模型组30只采用高脂饲料喂养,建立非酒精性脂肪肝模型。在第8周进行体质量、肝指数、肝组织匀浆超氧化物歧化酶活性检测,观察血清谷丙转氨酶、谷草转氨酶、三酰甘油、胆固醇、高密度脂蛋白胆固醇及低密度脂蛋白胆固醇等生化指标变化,并进行病理学检查。结果与结论:(1)病理切片示,模型组小鼠肝组织见大油滴和微小脂滴,并散布全肝,肝细胞肿胀,胞质易见脂肪空泡,肝细胞明显炎性改变;(2)模型组小鼠体质量和肝指数明显高于对照组小鼠,差异有显著性意义(P<0.05);但肝组织匀浆超氧化物歧化酶活性明显降低,差异有显著性意义(P<0.05);(3)与对照组小鼠相比,模型组小鼠血脂指标中三酰甘油、胆固醇、低密度脂蛋白胆固醇均有明显增高,但高密度脂蛋白胆固醇明显减低,差异有显著性意义(P<0.05);(4)结果提示通过高脂饮食诱导的非酒精性脂肪肝小鼠模型是理想的动物模型,方法简单,重复性强,可为非酒精性脂肪肝的机制研究及药物治疗提供稳定的动物模型。
BACKGROUND： The establishment of a safe, reliable and easily repeatable mouse model of nonalcoholic fatty liver disease is the prerequisite for the study of the diagnosis and treatment of the disease. OBJECTIVE： To establish a C57BL/6 mouse model of nonalcoholic fatty liver disease and observe changes of biochemical indicators, which can provide a theoretical basis for its pathogenesis and drug treatment. METHODS： Sixty healthy male C57BL/6 mice were randomly divided into a control group of 30 cases（normal diet）, and a model group of 30 cases（high fat diet）. Models of nonalcoholic fatty liver were established. At 8 weeks, body mass, liver index, and homogenate superoxide dismutase activity in the liver were detected. Changes in serum alanine aminotransferase, aspartate aminotransferase, triglyceride glycerol, cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol were observed. Pathological examination was performed. RESULTS AND CONCLUSION：（1） Pathological sections showed that large droplets and small lipid droplets in the mouse liver and spread the whole liver. Swelling of the liver cells, visible cytoplasmic vacuoles and obviously inflammatory changes in liver cells were observed in the model group.（2） Body weight and liver index were significantly higher in the model group than in the control group（P〈0.05）. Superoxide dismutase activity was significantly reduced in the liver（P〈0.05）.（3） Triglycerides, cholesterol, and low-density lipoprotein cholesterol levels were significantly higher, but high-density lipoprotein cholesterol levels were significantly lower in the model group than in the control group（P〈0.05）.（4） Nonalcoholic fatty liver mouse model is ideal for high-fat diet-induced animal model. The method is simple, repetitive, and can provide a stable animal model for the study on the mechanism of nonalcoholic fatty liver disease and drug treatment.
出处
《中国组织工程研究》
CAS
北大核心
2016年第40期6054-6059,共6页
Chinese Journal of Tissue Engineering Research
基金
河北省中医药管理局科研计划项目(2015290)~~
