摘要
目的:观察中药心康方对心力衰竭大鼠心肌miR-25-3p表达水平和肌浆网钙ATP酶2a(SERCA2a)活性的影响。方法:采用阿霉素腹腔注射法建立大鼠心力衰竭模型,随机分为正常对照组、假手术组、模型组、心康方组和卡托普利组,分别以蒸馏水、心康方和卡托普利连续灌胃35 d。观察大鼠心功能指标,ELISA法检测血浆脑钠肽水平,定磷法测定心肌SERCA2a活性,Western blot法检测心肌SERCA2a和受磷蛋白(PLB)的蛋白表达水平,茎环引物实时定量PCR检测心肌miR-25-3p的表达水平。结果:心康方组和卡托普利组大鼠心输出量、左室短轴缩短率和射血分数明显高于模型组,血浆脑钠肽水平明显低于模型组,miR-25-3p表达水平明显低于模型组,SERCA2a和PLB的蛋白表达水平明显高于模型组(P<0.01)。心康方组SERCA2a/PLB比值和SERCA2a活性明显高于模型组(P<0.05),卡托普利组与模型组比较无明显差异。结论:中药心康方可以改善心力衰竭大鼠心功能,机制可能与下调心肌miR-25-3p表达水平、提高SERCA2a的蛋白表达水平及增强SERCA2a活性有关。
AIM：To investigate the effects of Xinkang recipe on myocardial miR-25-3p expression and sarcoplasmic reticulum calcium ATPase 2a （ SERCA2a） activity in heart failure rats .METHODS：Male SD rats were randomly divided into normal group , sham group , model group , Xinkang recipe group （ Xinkang group ） , and captopril group .The heart failure rat model was induced by intraperitoneal injection of doxorubicin .Distilled water , Xinkang recipe and captopril were administrated by gastric gavage for 35 d, respectively .The indexes of cardiac function and plasma level of brain natriuretic peptide （BNP） were measured.The SERCA2a activity was determined by the inorganic phosphorus method . The myocardial protein expression of SERCA 2a and phospholamban （ PLB） was detected by Western blot .The myocardial expression of miR-25-3p was detected by stem-loop RT-qPCR.RESULTS：Cardiac output （CO）, left ventricular fractional shortening （ LVFS） and left ventricular ejection fraction （ LVEF） in Xinkang group and captopril group were significantly higher while the plasma levels of BNP were significantly lower than those in model group （P〈0.01）.The myocardial expression levels of miR-25-3p in Xinkang group and captopril group were significantly lower while the myocardial protein levels of SERCA2a and PLB were significantly higher than those in model group （P〈0.01）.The SERCA2a/PLB ratio and SERCA2a activity in Xinkang group were significantly higher than those in model group （P〈0.05）, and no significant change was observed between captopril group and model group .CONCLUSION：Xinkang recipe therapy may improve cardiac function in heart failure rats , which may be related to inhibiting the expression of miR-25-3p, increasing the SER-CA2a/PLB ratio and enhancing SERCA 2a activity in the myocardium .
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第10期1770-1774,共5页
Chinese Journal of Pathophysiology
