摘要
[目的]通过优化CTAB提取法从少量的野山参须根提取DNA,使其保持野山参形态的完整。[方法]综合比较CTAB提取法及其他3种DNA提取法,筛选出较为适合少量药材DNA的提取方法。[结果]从5 mg人参根须提取DNA,栽培参DNA浓度为200-1 000ng/μL,野山参为50-100 ng/μL,电泳可检测到清晰的条带,满足浓度和纯度的要求。[结论]该研究采用改进的高盐SDS法能从5 mg微量人参须根材料中提取高质量DNA,满足浓度和纯度的要求,可作为其他药材微量组织提取DNA的参考方法。
[Objective] The aim was to optimize CTAB method for DNA extraction from a small amount of fibrous roots of wild ginseng to keep the integrity form of wild ginseng.[Method] CTAB extraction method and other three kinds of DNA extraction method were comprehensively compared, CTAB method which is suitable for DNA extraction from a small amount of medicinal herbs was selected.[Result] DNA was extrac-ted from 5 mg ginseng root, the concentration of cultivated ginseng DNA was 200-1 000 ng/μL, wild ginseng was 50-100 ng/μL, electropho-resis can detect clear strip, meet the requirements of the concentration and purity.[ Conclusion] The improved high salt SDS method can extract high quality DNA from 5 mg trace ginseng fibril material.This method meet the requirements of the concentration and purity, and can be the ref-erence method for extracting DNA from other medicinal materials.
出处
《安徽农业科学》
CAS
2016年第24期155-156,172,共3页
Journal of Anhui Agricultural Sciences
关键词
栽培参
野山参
DNA提取
Cultivated ginseng
Wild ginseng
DNA extraction
