摘要
目的探讨锌或黄芪甲苷单独或联合对镉致人支气管上皮细胞(Human bronchial epithelial cells.16HBE)DNA损伤的拮抗效果,为预防镉暴露对机体造成的遗传损伤提供理论依据。方法采用单细胞凝胶电泳(single-cellgel electrophoresis,SCGE;又称彗星试验,comet assay)技术和CASP软件分析方法,检测不同剂量的锌或黄芪甲苷单独或联合对镉致16HBE细胞DNA损伤的拮抗效果。结果染毒及药物处理8周后,低、中剂量锌组或低剂量黄芪甲苷组的细胞损伤程度指数、拖尾率、彗星尾长、尾部DNA百分含量、尾矩和Oliver尾矩均数分别为1 574、1 417、1 447;87.8%、74.6%、81.4%;(12.74±7.33)μm、(12.26±7.04)μm、(17.98±14.16)μm;(23.40±15.71)%、(23.85±17.35)%、(23.28±21.46)%;(3.81±3.84)μm、(3.78±3.78)μm、(6.09±8.15)μm;(2.81±2.23)μm、(2.74±2.26)μm、(4.18±5.00)μm,而镉染毒16HBE细胞组(阳性组)分别为2 204、96.9%、(22.43±13.32)μm、(38.56±20.40)%、(12.62±11.63)μm、(7.27±6.26)μm,均明显高于上述各组(P<0.05)。中、高剂量黄芪甲苷组和低、中、高剂量联合组的细胞损伤强度指数分别为1 678、1 685、1 758、1 686、2 084,与阳性组比较,差异无统计学意义(P>0.05)。结论锌或黄芪甲苷对镉致正常16HBE细胞的DNA损伤均具有明显的拮抗作用,而两者的联合则使这种拮抗作用减弱。
Objective To study the antagonism of zinc or astragaloside alone or combination against cadmium-inducedDNA injury of normal 16 HBE cells,we provided the theoretical evidence for the prevention of cadmium- exposed geneticdamage of the body. Methods The antagonism of zinc or astragaloside alone or combination against cadmium-induced DNAinjury of 16 HBE cells under the condition of different doses was detected by using the single-cell gel electrophoresis（SCGE,also called comet assay）technology,and the results were analyzed with CASP software. Results The means of cells＇ DNAdamage indexes,tailing rates,tail lengths,percentages of tail DNA,tail moments and Oliver tail moments of the low and middledose zinc groups and low dose astragaloside group respectively were 1 574,1 417,and 1 447; 87.8%,74.6%,and 81.4%;（12.74±7.33）μm,（12.26±7.04）μm,and（17.98±14.16）μm;（23.40±15.71）%,（23.85±17.35）%,and（23.28±21.46）%;（3.81±3.84）μm,（3.78±3.78）μm,and（6.09±8.15）μm;（2.81±2.23）μm,（2.74±2.26）μm,and（4.18±5.00）μm,and those in the16 HBE cell group exposed to cadmium were respectively 2 204,96.9%,（22.43±13.32）μm,（38.56±20.40）%,（12.62±11.63）μm,and（7.27±6.26）μm,and the means of the low and middle dose zinc groups and low dose astragaloside group were lowerthan those of the 16 HBE cell group exposed to cadmium（P〈0.05）. The damage intensity indexes of cells in the middle andhigh dose astragaloside groups and in the low,middle and high dose combination groups were respectively 1 678,1 685,1 758,1 686,and 2 084,and there were no significant differences between those groups and the 16 HBE cell group exposed tocadmium,respectively（P〉0.05）. Conclusion Zinc or astragaloside has an obvious antagonism against normal 16 HBE cells＇ DNA damage induced by cadmium,but the combination of both zinc and astragaloside can weaken this antagonistic effect.
作者
傅晔
周小柳
刘可平
刘汉萍
王立辉
陈永忠
魏莲
雷毅雄
FU Ye ZHOU Xiao-liu LIU Ke-ping LIU Han-ping WANG Li-hui CHEN Yong-zhong WEI Lian LEI Yi-xiong(School of Public Health,Guangzhou Medical College, Guangdong 510100,China)
出处
《中国热带医学》
CAS
2016年第9期855-860,865,共7页
China Tropical Medicine
基金
国家自然科学基金项目(No.81573177)
广东省科技计划项目(No.2013B021800095)
广州市高等学校教育教学改革项目(No.20130285-Q149153)
广州医科大学教育科学规划项目(No.20130122-L149002)
广州医科大学"十二五"本科教学"质量工程"项目(No.B149041)
广州医科大学大学生科技创新项目(No.77)
关键词
锌
黄芪甲苷
氯化镉
人支气管上皮细胞
DNA损伤
拮抗
Zinc
Astragaloside
Cadmium chloride
Human bronchial epithelial cell
DNA damage
Antagonism
