摘要
目的优化天冬中皂苷的超声提取工艺,为质量检测提供基础。方法以紫外可见分光光度法测定天冬中皂苷的含量,并比较菝葜皂苷元与薯蓣皂苷元对照品与天冬样品的最大吸收波长,采用单因素实验法及Box-Benhken响应面法设计天冬皂苷的超声提取实验,并对Box-Benhken响应面模型进行方差分析。结果薯蓣皂苷元的最大吸收波长与天冬样品最大吸收波长相近。响应面分析的最佳提取条件为:乙醇浓度为48%,液料比为50mL/g,超声时间为61min。天冬皂苷的含量预测值为5.10%,验证试验的天冬皂苷含量的平均值为5.07%。结论选择薯蓣皂苷元作为对照品,可以较好地反映天冬皂苷含量水平。响应面法建立的天冬皂苷含量模型具有良好的实际指导意义,可作为进一步研究天冬的实验基础。
Objective To optimize the ultrasonic extraction of saponins from Asparagus cochinchinensis, and to provide a basis for quality evaluation. Methods The content of total saponins in Asparagus cochinchinensis was determined by ultraviolet spectrophotometry, and the maximum absorption wavelengths of sarsasapogenin, diosgenin, and Asparagus cochinchinensis were compared. The single-factor experiment and response surface methodology with Box-Behnken design were used to design the ultrasonic extraction experiment for saponins from Asparagus co- chinchinensis. An analysis of variance was performed for the model of the response surface methodology with Box- Behnken design. Results The maximum absorption wavelength of diosgenin was similar to that of Asparagus co- chinchinensis. The optimal extraction conditions by the response surface method were as follows： an ethanol concentration of 48% ,a liquid-solid ratio of 50 mL/g, and an ultrasonic extraction time of 61 min. The predicted content of saponins in Asparagus cochinchinensis was 5. 10%. The average content of saponins in Asparagus cochinchinensis in verification test was 5.07%. Conclusion Diosgenin is a good reference substance to reflect the content of saponins in Asparagus cochinchinensis. The saponins content model established by response surface methodology has a good guiding significance and can be used as the foundation of further research on Asparagus cochinchinensis.
出处
《安徽中医药大学学报》
2016年第5期89-93,共5页
Journal of Anhui University of Chinese Medicine
基金
国家中医药管理局中医药行业科研专项(201507002-2)
安徽高校科研创新平台团队项目(皖教秘科〔2015〕49号)
关键词
天冬
天冬皂苷
响应面
超声提取
Asparagus cochinchinensis
Saponin
Response surface
Ultrasonic extraction
