摘要
建立测定大鼠血浆中氧化槐定碱的体内分析方法,并考察其药代动力学参数。血浆样品以甲醇溶液沉淀,采用ZORBAX XDB-C18（150 mm×4.6 mm,4.0μm）色谱柱分离,流动相为乙腈-磷酸缓冲溶液（0.01 mol/L KH2PO4,H3PO4调p H 3.30）=4∶96（V/V）,检测波长210 nm。血浆中氧化槐定碱在2.50~500.00μg/m L内线性较好（r=0.9971）,提取回收率为77.3%~89.9%,日内、日间精密度RSD均小于10%。大鼠静脉注射50mg/kg和100 mg/kg氧化槐定碱后,其消除半衰期t1/2分别为（37.24±9.27）和（34.25±8.82）min,AUC分别为（1998.76±178.35）和（5130.62±1450.86）mg/L/min,Cmax分别为（89.53±10.47）和（247.35±112.02）mg/L。本文建立的方法简便、经济,可用于氧化槐定碱体内药代动力学分析。
To establish a method for the determination of oxysophoridine in rats plasma by HPLC-UV and to explore its profiles of pharmacokinetics. Plasma samples were extracted with methanol and separated on a ZORBAX XDB-C18（ 150 mm × 4. 6 mm,4 μm） column with a mobile phase consisting of phosphate buffer（ p H 3. 00） and acetonitrile（ 96∶ 4,v / v）. The detection wavelength was 210 nm. The assay was linear in the range of 2. 50 ~ 500. 00 μg / m L（ r = 0. 9974）.The recovery of oxysophoridine from plasma was 77. 3% to 89. 9%,and RSDs of inter-day and intra-day were less than10%. After intravenous injection of oxysophoridine（ 50,100 mg / kg） to rats,its t1 /2,AUC and Cmaxwere（ 37. 24 ±9. 27） and（ 34. 25 ± 8. 82） min,（ 1998. 76 ± 178. 35） and（ 5130. 62 ± 1450. 86） mg / L / min,and（ 89. 53 ± 10. 47）and（ 247. 35 ± 112. 02） mg / L,respectively. The method was convenient and economical. It was suitable for the study on pharmacokinetics of oxysophoridine in vivo.
作者
文友民
纪红燕
邓宁
王欣瑜
高华
WEN You-rain JI Hong-yan DENG Ning WANG Xin-yu GAO Hua(Department of Pharmacy, General Hospital of Ningxia Medical University, Yinchuan 750004, Chin)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2016年第9期1449-1453,共5页
Natural Product Research and Development
基金
宁夏医科大学青年基金(XQ2012012)
