摘要
目的：建立复方甘草口服溶液中甘草酸的含量测定方法。方法：采用高效液相色谱法（HPLC）,色谱柱为Agilent Eclipse Plus-C18（250 mm×4.6 mm,5μm）,流动相为甲醇-0.1%磷酸水溶液（70︰30）,检测波长250 nm,柱温25℃,流速1.0 m L/min,进样量20μL。结果：甘草酸浓度在29.18~182.35μg/m L范围内时,样品浓度与其相应色谱峰峰面积积分值呈良好线性关系（r=0.999 2）。精密度、稳定性和重复性试验的相对标准偏差（RSD）分别为0.59%、0.66%和0.40%;样品平均加样回收率为97.47%,RSD为1.49%（n=9）。结论：该方法专属性、重复性和准确性均较好,可用于复方甘草口服溶液中甘草酸的含量测定。
Objective： To establish a method of quantitative determination of glycyrrhizic acid in compound glycyrrhiza oral solution. Methods： HPLC analytic method was used. The Agilent Eclipse Plus-Cls column （250 mm ×4. 6mm, 5 μm） was used at room temperature （25 ℃ ） and eluted by methanol-0. 1% phosphoric acid solution （70 ： 30） with a flow rate of 1.0 mL/min. The detection wavelength was 250 nm and the injection volume was 20 μL. Results： A good linear relationship was obtained between the peak areas and the concentrations of glycyrrhizic acid ranging from 29. 18 - 182. 35 μg/mL （ r = 0. 999, 2 ）. RSDs for precision, stability and reproducibility tests were 0. 59% , 0.66% and 0.40% , respectively. The average recovery was 97.47% （ RSD = 1.49%, n = 9 ）. Conclusion： The HPLC method which was specific, accurate and reproducible could be used for quantitative determination of glycyrrhizic acid in compound glycyrrhiza oral solution.
出处
《儿科药学杂志》
CAS
2016年第10期51-53,共3页
Journal of Pediatric Pharmacy
基金
南京医科大学科技发展基金项目
编号2012NJMU068
关键词
复方甘草口服溶液
甘草酸
高效液相色谱法
含量测定
compound glycyrrhiza oral solution
glycyrrhizie acid
HPLC
quantitative determination
