摘要
目的建立高效液相色谱法（HPLC ）法测定清火胶囊中芦荟大黄素、大黄素和大黄酚含量的方法.方法：采用 Eclipsc XDB（ 4. 6 mm× 250 mm , 5 μm ）色谱柱; 流动相为甲醇-0. 1 %磷酸溶液（80：20）, 流速为0. 8 0 mL /min, 检测波长：254 nm温30°C .结果：芦荟大黄素、大黄素和大黄酚与其相邻质峰能完全分离.芦荟大黄素、大黄素和大黄酚的线性范围分别0.63-3. 5μg /mL （ r =0.999 9）、0.34-17. 2μg /mL （ r =1.000 0）、0.72-36. 0μg /mL （ r =0.999 8）.方法回收率均不于97%.结论：本方法简便、准确、重现性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价.
Objective To establish HPLC method for quantification of aloe-emodin, emodin and chry- sophanol in Qinghuo capsule. Methods： HPLC column was Eclipse XDB （4. 6 mm×250 mm, 5 μm） with methanol-0. 1 % phosphoric acid （80 ： 20） as mobile phase. The flow rate was set to 0. 8 mL/min and the wavelength for detection was 254 nm. The column temperature was 30C. Results： Peaks of aloe-emodin, emodin and chrysophanol could be completely isolated from other impurities. The linear range for aloe-emodin, emodin and chrysophanol were 0. 63 to 31. 5μg/mL （ r = 0. 999 9） ,0. 34 to 1 7. 2 μg /mL （ r = 1. 000 0） and 0. 72 to 36. 0 0μg/mL （ r = 0. 999 8）, respectively. The recovery rates of all three compounds were not less than 97%. Conclusion： The HPLC method established here is simple, reliable and reproduti- ble,which can be used in the quality control of Qinghuo capsule.
作者
申伟培
曾铮
Shen Weipei Zeng Zheng.(Department of Pharmacology, the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2016年第5期899-902,共4页
Journal of Guangxi Medical University
