地塞米松对急性肺损伤中TREM-1表达的影响被引量：6

Effect of dexamethasone on the expression of TREM-1 in acute lung injury

导出

摘要目的探讨地塞米松(Dexamethasone,Dex)对脂多糖(Lipopolysaccharide,LPS)诱导的急性肺损伤(acute lung injury,ALI)小鼠肺组织中髓样细胞表达的触发受体1(triggering receptor expressed on myeloid cells-1,TREM-1)表达的影响。方法以昆明小鼠为研究对象,腹腔注射LPS(10 mg/kg)建立ALI模型,30 min后给予不同浓度的Dex(5、10、20、40 mg/kg)处理6 h;选用Dex(10 mg/kg)处理不同的时间(6、12、24、36 h)。HE染色法观察肺组织病理损伤程度;RT-PCR检测肺组织TREM-1mRNA的表达;ELISA检测小鼠支气管肺泡灌洗液中可溶性TREM-1(s TREM-1)蛋白水平。结果 Dex可减轻肺病理损伤;Dex呈时间依赖性地下调ALI小鼠肺组织的TREM-1 mRNA的表达,且在6 h即可降低;Dex呈剂量依赖地下调ALI小鼠肺组织中TREM-1 mRNA的表达,并在10 mg/kg时开始降低。Dex可降低ALI小鼠支气管肺泡灌洗液中sTREM-1的蛋白水平。结论 Dex可呈剂量及时间依赖性下调ALI小鼠肺组织中的TREM-1mRNA的表达,并减少肺内髓样细胞胞膜TREM-1的脱落,提示Dex可能通过调节TREM-1的表达,从而抑制ALI早期的炎症级联反应,参与保护肺组织。 Objective To investigate the effects of dexamethasone （Dex） on the TREM-1expression in acute lung injury（ALI） of mice induced by lipopolysaccharide （LPS）. Methods Kunming mice （KM mice） were used as the research object and LPS （10 mg/kg） was used to induce acute lung injury model. After half an hour given different concentrations of Dex （5, 10, 20, 40 mg/kg） and selection of different time points （6, 12, 24 and 36 h） were observed. The lung tissue was observed by HE staining, RT-PCR was used to detect TREM-1 expression, and ELISA was used to detect the expression of sTREM-1 in bronchoalveolar lavage fluid of mice. Results Dex could time-dependently down-regulate the expression of lung tissue in the acute lung injury and in 6h treatment peak;Dexcould dose-dependently down-regulate TREM-1 expression in acute lung injury of lung tissue, and reached the peak at 10 mg/kg. Dex could decrease the protein level of sTREM-1 in bronchoalveolar lavage fluid of ALI mice. Conclusion Dex can down-regulate TREM-1 of lung tissues of ALI mice in a dose and time-dependent manner, and reduce myeloid cell membrane of TREM-1 in lung. Our research indicates that Dex may inhibit inflammatory cascade and protect lung tissue by regulation of TREM-1 expression.

作者骆凤郑浩翔董良李年旺周琴贾哲贺丽萍孙国瑛

机构地区湖南师范大学医学院基础医学系组织学与胚胎学教研室湖南师范大学树达学院遵义医学院附属医院麻醉科中南大学基础医学院

出处《中国临床解剖学杂志》 CSCD 北大核心 2016年第5期523-527,共5页 Chinese Journal of Clinical Anatomy

基金湖南省教育厅科研项目(14C0703) 中南大学博士后基金(160320001) 2015年湖南师范大学树达学院创新课题(012-615)

关键词髓样细胞表达的触发受体-1 急性肺损伤地塞米松脂多糖 Triggering receptor expressed on myeloid cells-l Acute lung injury Dexamethasone Lipopolysaccharide

分类号 R563 [医药卫生—呼吸系统]

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参考文献14

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