摘要
目的:探讨亲环蛋白A(cyclophilin A,Cy PA)对氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)诱导小鼠巨噬细胞RAW264.7活化和凋亡的影响。方法:体外培养小鼠巨噬细胞RAW264.7,蛋白质印迹法检测40mg/m L ox-LDL诱导后RAW264.7细胞Cy PA的表达。转染siRNA沉默Cy PA的表达,转染阴性对照siRNA作为阴性对照组,使用40 mg/L ox-LDL处理细胞24 h后,通过流式细胞术检测各组巨噬细胞表面标志CD80和CD86的阳性率、细胞凋亡率。结果:与空白对照组相比,ox-LDL干预24 h后RAW264.7细胞Cy PA表达量显著增高。沉默Cy PA表达后,与阴性对照组相比,Cy PA沉默组在ox-LDL干预24 h后CD80、CD86的表达明显降低,细胞凋亡明显减少。结论:Cy PA可能参与介导ox-LDL诱导的RAW264.7细胞活化与凋亡。
Objective： To investigate the effect of cyclophilin A （CyPA） on ox-LDL-induced activation and apoptosis in RAW264.7 cells. Methods ： RAW26ZL 7 cells were incubated with 40 mg/L ox-LDL for 24 h, Western blotting analysis was employed to detect the CyPA expression. After transfected with CyPA siRNA, the cells were incubated with 40 mg/L ox-LDL for 24 h. The positive rate of CD80, CD86 and the apoptosis rate were determinated by flow cytometry analysis. Results： After incubating with ox-LDL, the level of CyPA was upregulated. The percentage of CDS0 positive cells, CD86 positive cells and apoptotic cells were significantly decreased in CyPA knockdown cells compared with the negative control siRNA cells after the intervention of ox-LDL. Conclusion： CyPA may mediate the ox-LDL-induced activation and apop- tosis in RAW264.7 cells.
出处
《江苏大学学报（医学版）》
CAS
2016年第2期128-131,共4页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金资助项目(81370409
81200222)
江苏省卫生厅重点项目(H201435)
江苏省333工程人才基金资助项目(BRA2015208)
镇江市社会发展项目(SH2015035)
